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Further Report on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotypes |
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| Authors: | ZHANG De-Li XIA Geng-tian HE Xu-yu GAO Bu-xian BAI Xiao-hong LI Liu-jin HUANG Gao-sheng LIU Shang-gao YEN Lung-fei FANG Fu-de |
| Institution: | 1. Peking University Health Science Center, Beijing 100083,P.R.China;Beijing Institute of Preventive Medicine, Beijing 100071, P.R.China;Center for Research and Development of Animal Healthcare Products, Fourth Military Medical University, Xi'an 710032, P.R. 2. Beijing Institute of Preventive Medicine, Beijing 100071, P.R.China 3. Center for Research and Development of Animal Healthcare Products, Fourth Military Medical University, Xi'an 710032, P.R.China 4. Institute for Cancer Research at China Cancer Research Foundation, Fourth Military Medical University, Xi'an 710032, P.R.China 5. Key Open Laboratory of Preventive Veterinary Medicine, Ministry of Agriculture/China Agricultural University, Beijing 100094, P.R.China 6. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing 100005, P.R.China |
| Abstract: | Using Hela cell cultures as positive control and primary canine kidney cell (CKC) or feline kidney cell (FKC) cultures purified in vitro on passage 3 as negative control, the tumorigenicity of Madin-Darby canine kidney (MDCK) cells was tested in ＞273 nude mice, and colony formation in soft agarose and haemagglutination under different concentration of plant lectins of these cells were carried out at the same time. Subsequently, very low tumorigenicity strains of MDCK line were successfully selected; these were evaluated for the production of canine or feline combination viral vaccines, free of infectious agents, and of known cytogenetic and tumorigenic. It is thus evident that MDCK cell of M, JB, JC, WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA, YB and KA strains can not be approved as substrate for the preparation of attenuated viral vaccines. The heritable character of these cell sub-lines is comparatively stable, and shows little significant difference between passages. |
| Keywords: | Kidney cell line Nude mice Carcinogenesis Tumorigenicity Canine kidney cell (CKC) MDCK |
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