Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey |
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| Authors: | Gül?en Sertkaya Marta Martini Paolo Ermacora Rita Musetti Ruggero Osler |
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| Institution: | (1) Dept. of Plant Protection, Agriculture Faculty, Mustafa Kemal University, 31034 Antakya, Turkey;(2) Dept. of Biologia Applicata alla Difesa delle Piante, University of Udine, 33100 Udine, Italy |
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| Abstract: | During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases
in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear
trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and
plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases.
Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European
stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in
Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY
phytoplasma infecting Japanese plum was previously reported.
http://www.phytoparasitica.org posting July 21, 2005. |
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| Keywords: | Pear phytoplasma polymerase chain reaction restriction fragment length polymorphism stone fruit transmission electron microscopy |
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