Identification and Characterisation of Bacteria Causing Soft-rot in Agave tequilana |
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| Authors: | I Jiménez-Hidalgo G Virgen-Calleros O Martínez-de la Vega G Vandemark V Olalde-Portugal |
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| Institution: | (1) Laboratory of Plant Pathology, Faculty of Agriculture, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-Ku, Shizuoka 422-8529, Japan;(2) Kochi Agricultural Research Center, Hataeda, Kochi 783-0023, Japan;(3) Tottori Agricultural Experiment Station, 260 Hashimoto, Tottori 680-1142, Japan;(4) Laboratory of Plant Pathology and Biotechnology, Kochi University, Nankoku, Kochi 783-8502, Japan; |
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| Abstract: | Agave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms. |
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| Keywords: | Agave tequilana Erwinia cacticida Pantoea agglomerans Pseudomonas sp soft-rot |
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