Genetic diversity of <Emphasis Type="Italic">Guizotia abyssinica</Emphasis> (L. f.) Cass. (Asteraceae) from Ethiopia as revealed by random
amplified polymorphic DNA (RAPD) |
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| Authors: | Mulatu Geleta Tomas Bryngelsson Endashaw Bekele Kifle Dagne |
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| Institution: | (1) Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, Alnarp, SE 230 53, Sweden;(2) Department of Biology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia |
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| Abstract: | Genetic diversity of 70 populations of niger (Guizotia abyssinica) representing all its growing regions in Ethiopia was investigated using random amplified polymorphic DNA (RAPD) to reveal
the extent of its populations genetic diversity. Ninety-seven percent of the loci studied was revealed to be polymorphic for
the whole data set. The within population diversity estimated by Shannon diversity index and Nei gene diversity estimates
was revealed to be 0.395 and 0.158, respectively. The extent of genetic variation of populations from major niger producing
regions was significantly lower than that of populations from other regions; however, it is distributed regardless of altitude
of growth. Genetic differentiation between populations was estimated with Shannon index as G′
ST (0.432), Nei’s G
ST (0.242) and AMOVA based F
ST (0.350) and appears to be equivalent to the average values calculated from various RAPD based studies on outcrossing species.
Higher proportion of the variation detected by AMOVA resided within populations (64.58%) relative to the amount of variation
among populations (35.42%). UPGMA cluster analysis showed that most of the populations were clustered according to their region
of origin. However, some populations were genetically distant from the majority and seem to have unique genetic properties.
It is concluded that the crop has a wide genetic basis that may be used for the improvement of the species through conventional
breeding and/or marker assisted selection. Collection of germplasm from areas not yet covered and/or underrepresented is the
opportunity to broaden the genetic basis of genebank collection. |
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| Keywords: | AMOVA Genetic diversity Germplasm Guizotia abyssinica Niger Population RAPD |
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