| 栽培稻种内rDNA基因IGS序列分析及系统学意义 |
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| 引用本文： | 戴小军,欧立军,李文嘉,梁满中,陈良碧.栽培稻种内rDNA基因IGS序列分析及系统学意义[J].作物学报,2008,34(9):1569-1573. |
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| 作者姓名： | 戴小军 欧立军 李文嘉 梁满中 陈良碧 |
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| 作者单位： | 湖南师范大学生命科学学院, 湖南长沙410081 |
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| 摘 要： | 以普通野生稻为对照, 将rDNA基因间区(IGS)序列用于栽培稻种内不同亚种以及亚种内不同品种的亲缘关系分析。结果表明, 栽培稻种内IGS序列长度为2 130~2 145 bp, G+C含量为74.59%~75.29%, 变异位点229个, 占10.70%, 信息位点76个, 占3.51%。IGS序列中籼亚种和粳亚种之间有38个亚种标志性碱基差异, 主要分布在IGS 5′ 端近400 bp的序列中。用IGS序列构建的系统树能将栽培稻的籼亚种和粳亚种分为两大类, 亚种内不同亲缘关系的品种也能区分开。本研究结果支持爪哇稻为栽培稻中一个独立亚种的观点。
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| 关 键 词： | 栽培稻 核糖体 基因间区(IGS) 系统树 |
| 收稿时间： | 2007-12-03 |
| 修稿时间： | 1900-01-01 |
Analysis of rDNA Intergenic Spacer (IGS) Sequences in Oryza sativa L. and Their Phylogenetic Implications |
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| DAI Xiao-Jun,OU Li-Jun,LI Wen-Jia,LIANG Man-Zhong,CHEN Liang-Bi.Analysis of rDNA Intergenic Spacer (IGS) Sequences in Oryza sativa L. and Their Phylogenetic Implications[J].Acta Agronomica Sinica,2008,34(9):1569-1573. |
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| Authors: | DAI Xiao-Jun OU Li-Jun LI Wen-Jia LIANG Man-Zhong CHEN Liang-Bi |
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| Institution: | College of Life Science, Hunan Normal University, Changsha 410081, Hunan, China |
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| Abstract: | In plant unclear genome, the intergenic spacer region (IGS) is located on the region of 18S–28S and is often used in phylogenetic analysis among species, detecting intra-specific polymorphisms. Rice (Oryza sativa L.) is differentiated into not only some subspecies but also many ecotypes of these subspecies. Can the IGS region be a useful tool to study the phylogenetic relationships among these cultivars of Oryza sativa? In the study, a comparative analysis of the rDNA intergenic spacer entire sequences of thirteen cultivars in Oryza sativa L. was carried out with O. rufipogon as outgroup. In the cultivars used, there were six for typical O. sativa ssp. japonica, five for typical O. sativa ssp. indica, two for O. sativa ssp. javanica. The result indicated that the length range of IGS sequences was from 2 130 to 2 145 bp, the content of G+C varied from 74.59% to 75.29%, there were 229 variational loci which were 10.70% of entire bases and 76 informative loci which were 3.51% of entire bases in Oryza sativa. Thirty-eight indica and japonica specific loci were found in the IGS sequences of Oryza sativa, most of them were covered a about 400 bp segment located in the upstream of IGS sequence. The indica and japonica subspecies of Oryza sativa, as well as some closely related cultivars, can be discriminated clearly based on the phylogenetic tree constructed by IGS sequences. The study supported the opinion that the O.sativa ssp.javanica is a subspecies of Oryza sativa. |
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| Keywords: | Rice (Oryza sativa L ) Ribosomal DNA (rDNA) Intergenic spacer (IGS) Phylogenetic tree |
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