| 玉米愈伤组织的遗传转化和对草丁膦抗性及植株再生的研究 |
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| 引用本文： | 姚丹, 王丕武, 刘占柱, 郝文媛, 关淑艳.玉米愈伤组织的遗传转化和对草丁膦抗性及植株再生的研究[J].玉米科学,2004,12(3):033-035. |
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| 作者姓名： | 姚丹 王丕武 刘占柱 郝文媛 关淑艳 |
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| 作者单位： | 吉林农业大学生物中心,吉林,长春,130118 |
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| 基金项目： | "国家植物转基因中试及产业化基地"专项课题(J99-B-001) |
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| 摘 要： | 以玉米自交系7922、P2和H99的胚性愈伤组织为材料,通过愈伤组织对草丁膦的敏感性实验,确定愈伤组织适宜选择压,并探讨了不同激素对愈伤组织分化的影响,用带有质粒PGBIloxAp.Bt.35sp.bar的根癌农杆菌LBA4404转化玉米愈伤组织,PCR检测证明,目的基因已整合到玉米基因组中。
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| 关 键 词： | 玉米 自交系 愈伤组织 农杆菌 遗传转化 草丁膦 |
| 文章编号： | 1005-0906(2004)03-0033-03 |
| 收稿时间： | 2003/8/13 0:00:00 |
| 修稿时间： | 2003年8月13日 |
Maize Embryogenic Calli Transformation and Resistance to Herbicide Basta and Plant Regeneration |
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| YAO Dan; WANG Pei-wu; LIU Zhan-zhu; Wen-yuan; GUAN Shu-yan.Maize Embryogenic Calli Transformation and Resistance to Herbicide Basta and Plant Regeneration[J].Journal of Maize Sciences,2004,12(3):033-035. |
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| Authors: | YAO Dan; WANG Pei-wu; LIU Zhan-zhu; Wen-yuan; GUAN Shu-yan |
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| Abstract: | The embrogenic callus of inbred 7922, P2 and H99 were selected on MS medium containing different concentration of PPT, after 30 days the result of tissue culture indicated that 6 mg/L PPT was the most suitable selected concentration:and in this study we found that maize callus were more suitable to regeneration plant on MS medium containing 6-BA 1 mg/L and KT 2 mg/L. Bt gene was successfully transferred into callus through agrobacterium tumefaciens LBA4404(PACT). The analysis of PCR demonstrated that the extrogenous gene had integrated into the maize genome. |
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| Keywords: | Maize: Inbred: Embryogenic calli: Agrobacterium tumefaciens: Transformation: Basta |
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