| 玉米大斑病菌人工接种方法研究 |
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| 引用本文： | 刘烨珏,张敏,伍智华,李静英.玉米大斑病菌人工接种方法研究[J].植物病理学报,2012,42(1):101-104. |
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| 作者姓名： | 刘烨珏 张敏 伍智华 李静英 |
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| 作者单位： | 四川农业大学农学院植物病理系，雅安 625014 |
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| 基金项目： | 国家玉米产业技术体系建设项目 |
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| 摘 要： | The efficient and accurate method of inoculating Exserohilum turcicum on maize is most important for studying the corn leaf blight. In this study, four approaches including spore suspension spraying, inoculation with grinding infected maize leaves, inoculation liquid inoculum into the leaf whorl and inoculation with inoculated sorghum seeds were compared with natural induction in fields during different growth stages of maize in the greenhouse and field. The results showed that disease incidence of all varieties tested were up to 100% by inoculating with inoculated sorghum seeds during the booting stage (11 to 12 leaf stages). This method will not only guarantee the enough pathogen inocula but also be easy to use, and could be an effective way for disease resistance identification in field and greenhouse.
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| 关 键 词： | maize Exserohilum turcicum inoculation method |
Artificial inoculation methods for Exserohilum turcicum on maize |
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| LIU Ye-jue
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ZHANG Min
,
WU Zhi-hua
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LI Jing-ying.Artificial inoculation methods for Exserohilum turcicum on maize[J].Acta Phytopathologica Sinica,2012,42(1):101-104. |
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| Authors: | LIU Ye-jue ZHANG Min WU Zhi-hua LI Jing-ying |
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| Institution: | Department of Plant Pathology, Sichuan Agricultural University, Ya′an 625014, China |
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| Abstract: | The efficient and accurate method of inoculating Exserohilum turcicum on maize is most important for studying the corn leaf blight.In this study,four approaches including spore suspension spraying,inoculation with grinding infected maize leaves,inoculation liquid inoculum into the leaf whorl and inoculation with inoculated sorghum seeds were compared with natural induction in fields during different growth stages of maize in the greenhouse and field.The results showed that disease incidence of all varieties tested were up to 100% by inoculating with inoculated sorghum seeds during the booting stage(11 to 12 leaf stages).This method will not only guarantee the enough pathogen inocula but also be easy to use,and could be an effective way for disease resistance identification in field and greenhouse. |
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| Keywords: | maize Exserohilum turcicum inoculation method |
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