| 马铃薯转萝卜抗菌肽基因植株的获得及其抗晚疫病的初步鉴定 |
| |
| 引用本文： | 任玉霞,陈凌娜,刘桂珍,王晴,杨生牛,石春鸿,李婷婷,侯路珍,陈继峰,陈正华.马铃薯转萝卜抗菌肽基因植株的获得及其抗晚疫病的初步鉴定[J].中国蔬菜,2012,1(6):68-73. |
| |
| 作者姓名： | 任玉霞 陈凌娜 刘桂珍 王晴 杨生牛 石春鸿 李婷婷 侯路珍 陈继峰 陈正华 |
| |
| 作者单位： | 1.郑州大学生物工程系，河南郑州 450000,2甘肃亚盛集团博士后科研工作站，北京 100101,3北京林业大学生物科学与技术学院，北京 100083,4北京青神园林工程有限公司，北京 100012 |
| |
| 基金项目： | 甘肃省科技厅项目(1011NKCA079) |
| |
| 摘 要： | 为获得抗晚疫病的转基因马铃薯植株，采用根癌农杆菌介导法，以微型薯薯片为外植体，将萝卜抗菌肽基因（AFP）导入马铃薯栽培品种夏波蒂（Shepody）中，通过草甘膦抗性筛选和PCR检测获得了4株转基因试管苗，并经黑暗诱导获得了微型薯。采用离体叶片接种法，对微型薯长出的叶片进行抗晚疫病鉴定，结果表明这4个株系的抗病性均有一定程度的提高。
|
| 关 键 词： | 马铃薯 抗菌肽基因（AFP） 基因转化 晚疫病 |
Procurement of Transgenic Potato with AFP Gene and Preliminary Identification of Its Late Blight Resistance |
| |
| REN Yu-xia,CHEN Ling-na,LIU Gui-zhen,WANG Qing,YANG Sheng-niu,SHI Chun-hong,LI Ting-ting,HOU Lu-zhen,CHEN Ji-feng,CHEN Zheng-hua.Procurement of Transgenic Potato with AFP Gene and Preliminary Identification of Its Late Blight Resistance[J].China Vegetables,2012,1(6):68-73. |
| |
| Authors: | REN Yu-xia CHEN Ling-na LIU Gui-zhen WANG Qing YANG Sheng-niu SHI Chun-hong LI Ting-ting HOU Lu-zhen CHEN Ji-feng CHEN Zheng-hua |
| |
| Institution: | 1.Bio-engineering Department of Zhengzhou University，Zhengzhou 450000，Henan，China|2Postdoctoral Scientific Research Station of Gansu Yasheng Industrial（Group）Co.，Ltd.，Beijing 100101，China|3College of Life Sciences and Biotechnology，Beijing Forestry University，Beijing 100083，China|4Beijing Qingshen Landscape Engineering Co.，Ltd.，Beijing 100012，China |
| |
| Abstract: | The aim of the study is to obtain the transgenic potato(Solanum tuberosum L.)lines conferring resistance to late blight.AFP(antifungal protein)gene was introduced into potato susceptible cultivar’Shepody’via Agrobacterium tumefaciens-mediated transformation,using micro-tuber discs as explants.Four positive plantlets were obtained by PCR assay and the glyphosate selection.The micro-tubers were introduced in the dark and then planted in the field to grow into complete plants for the identification of disease resistance.Using detached leaf assay,4 transgenic plant lines were identified for late blight resistance.The result showed that their disease resistance was higher than the non-transgenic ones. |
| |
| Keywords: | Potato Antifungal protein(AFP) Genetic transformation Late blight |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 |
