| 鸡黄病毒CJD05株E基因克隆与序列分析 |
| |
| 引用本文： | 王劭,陈仕龙,陈少莺,林锋强,程晓霞,朱小丽,江斌,李兆龙.鸡黄病毒CJD05株E基因克隆与序列分析[J].中国预防兽医学报,2012,34(4):317-319. |
| |
| 作者姓名： | 王劭 陈仕龙 陈少莺 林锋强 程晓霞 朱小丽 江斌 李兆龙 |
| |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建福州350003;福建省畜禽疫病防治工程技术研究中心,福建福州350013
2. 福建省农业科学院畜牧兽医研究所,福建福州,350003 |
| |
| 基金项目： | 福建省农科院创新团队项目(STIF-Y02);福建省公益类科研院所专项(2011R1025-2) |
| |
| 摘 要： | 为了解福建省鸡黄病毒(CFV) CJD05株来源及其遗传进化关系,根据鸭黄病毒(DFV) BYD-1株E基因全序列设计合成1对引物,特异性扩增CFV CJD05株的E基因,并对其序列进行分析.结果表明克隆获得CFV CJD05株1 503 bp的E基因特异性目的条带,同源性分析表明CFV CJD05株E基因核苷酸序列与DFVBYD-1株、鹅黄病毒(GFV) JS804株的同源性分别为99.2％、99.3％,氨基酸的同源性分别为99.0％、98.6％,表明CFV CJD05株、DFV BYD-1株和GFV JS804株高度同源,与坦布苏病毒(TBSV)的同源性高于其它虫媒介黄病毒.
|
| 关 键 词： | 鸡黄病毒 E基因 序列分析 |
Cloning and sequence analysis of E gene of a chicken flavivirus isolate CJD05 in China |
| |
| WANG Shao
,
CHEN Shi-long
,
CHEN Shao-ying
,
LIN Feng-qiang
,
CHENG Xiao-xia
,
ZHU Xiao-Li
,
JIANG Bin
,
LI Zhao-long.Cloning and sequence analysis of E gene of a chicken flavivirus isolate CJD05 in China[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):317-319. |
| |
| Authors: | WANG Shao CHEN Shi-long CHEN Shao-ying LIN Feng-qiang CHENG Xiao-xia ZHU Xiao-Li JIANG Bin LI Zhao-long |
| |
| Institution: | 1(1.Institute of Animal Husbandry and Veterinary Medicine,FuJian Academy of Agricultural Sciences,Fuzhou 350003,China; 2.Fujian Animal Diseases Control Technology Development Center,Fuzhou 350013,China) |
| |
| Abstract: | To investigate the CJD05 chicken flavivirus(CFV) Fujian isolate and phylogenetic relationships,a pair of primers were designed and synthesized based on the sequences of the duck flavivirus(DFV) E gene,the E gene of CFV CJD05 was amplified by RT-PCR and cloned into pMDT-18 vector for sequencing.The results showed that the full length E gene was 1,503 bp,encoding a protein of 501 amino acids.Evolution analysis demonstrated that the E nucleotide sequence from isolate CJD05 shared 99.2% identity with DFV BYD-1 strain,99.3% with goose flavivirus(GFV) JS804 strain,and 99.0% and 98.6% amino acid sequence identities with DFV BYD-1 and GFV JS804 strain,respectively.Avian flavivirus is unusual in that it affects terrestrial bird and aquatic bird,the homology of E gene among avian flavivirus and Tembusu virus is higher than that among avian flavivirus and in other arthropod-borne flavivirus. |
| |
| Keywords: | chicken flavivirus E gene sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
