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巴马香猪精液冷冻保存研究
引用本文:马志宏.巴马香猪精液冷冻保存研究[J].畜牧兽医杂志,2013,32(5):3-4,6.
作者姓名:马志宏
作者单位:甘肃省畜牧兽医研究所,甘肃平凉,744000
摘    要:利用甘油和二甲基亚砜(DMSO)作为冷冻保护剂,以精子存活率为评价指标,研究了冷冻保护剂对香猪精液冷冻保存效果的影响。试验分工、Ⅱ、Ⅲ、Ⅳ4个试验组和1个对照组。以解冻后的精子活力、活率为判断标准,比较了4种冷冻保护液对香猪精液冷冻保存的影响。结果表明,试验Ⅰ、Ⅱ、Ⅳ组精子活率显著高于Ⅲ组(含5%甘油)(P〈0.05);试验Ⅰ、Ⅱ、Ⅳ组之间差异不显著(P〉0.05);Ⅰ组精子活率最高,达到53.10%。

关 键 词:巴马香猪  冷冻保存  活率

Study on Semen Cryopreservation Technique for Bama Miniature Pig
MA Zhi-hong.Study on Semen Cryopreservation Technique for Bama Miniature Pig[J].Journal of Animal Science and Veterinary Medicine,2013,32(5):3-4,6.
Authors:MA Zhi-hong
Institution:MA Zhi-hong ( Gansu Institute of Animal Science and Veterinary Medicine, P ingliang Gansu 744000, China)
Abstract:This study aimed to evaluate the effects of cryoprotective agent glycerol and dimethyl sulfoxide (DMSO) on mini- ature pig semen, with sperm survival rate as evaluation index. A control group and four test groups were contained in this stud- y. The results demonstrated that semen survival rates of test group 1,2 and 4 were significantly higher than test group 3 (3% glycerin) (P〈0.05). However, there was no significant difference among that three groups (P〉0.05). Survival rate of group 1 was the highest one,which reached 53.10%.
Keywords:Bama miniature pig  semen cryopreservation  motility  viability
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