Molecular genotyping by pulsed-field gel electrophoresis of restricted genomic DNA of strains of taylorella equigenitalis isolated in Ireland and in the United States |
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| Authors: | Matsuda M Miyazawa T Moore JE Buckley TC Thomas LA |
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| Institution: | (1) Laboratory of Molecular Biology, The Graduate School of Environmental Health Sciences, Azabu University, 1-17-71 Fuchinobe, Sagamihara, 229-8501, Japan;(2) Molecular Epidemiology Unit, Northern Ireland Public Health Laboratory, Belfast City Hospital, Belfast, BT9 7AD, Northern Ireland;(3) Irish Equine Centre, Johnstown, Nass, Co, Kildare, Ireland;(4) National Veterinary Services Laboratories, PO Box 844, Ames, IA, USA |
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| Abstract: | The profiles, after digestion with ApaI or NotI and pulsed-field gel electrophoresis (PFGE), of genomic DNA from 18 strains of Taylorella equigenitalis isolated in Ireland were of three different types. The 13 strains in one of these types gave PFGE profiles identical to that of an American prototype strain, Kentucky 188, but different from strain NCTC11184T and from a strain isolated in Japan.Eight additional strains isolated in the United States gave four distinct types of genomic PFGE profiles. All four types were different from that of T. equigenitalis NCTC11184T or that of the Japanese strain. The profile of three strains of one type was identical to that of Kentucky 188. |
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| Keywords: | contagious equine metritis genotype Ireland USA T equigenitalis pulsed-field electrophoresis |
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