| 玉米Rubisco活化酶基因ZmRCA1的序列变异分析 |
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| 引用本文： | 谭贤杰,宋燕春,石云素,程伟东,吴子恺,王天宇,黎裕.玉米Rubisco活化酶基因ZmRCA1的序列变异分析[J].作物学报,2011,37(1):58-66. |
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| 作者姓名： | 谭贤杰 宋燕春 石云素 程伟东 吴子恺 王天宇 黎裕 |
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| 作者单位： | 1 广西大学农学院，广西南宁530005; 2 广西农科院玉米所，广西南宁530227; 3 中国农业科学院作物科学研究所，北京100081 |
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| 基金项目： | 本研究由国家重点基础研究发展计划(973计划)项目(2011CB100100, 2009CB118401)和国家高技术研究发展计划(863计划)项目(2006AA10Z188)资助。 |
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| 摘 要： | Rubisco活化酶(RCA)是一种可激活光合作用关键酶Rubisco的伴侣蛋白，可通过对Rubisco的活性调节决定植物的碳同化效率。为了研究玉米ZmRCA1的多态性，本研究参考GenBank中编码玉米Rubisco活化酶的ZmRCA1基因组序列设计引物对玉米微核心种质的95份自交系的ZmRCA1进行测序，获得长约1 680 bp的基因组序列。多态分析表明，在1 680 bp的区间内共发现22个SNP和8个InDel，其中5个SNP和1个InDel变异产生氨基酸序列改变；频率在0.1以上的13个多态性位点共形成27种单倍型，利用6个多态性位点就可以分辨约90%的单倍型。ZmRCA1基因具有高度序列保守性，基因DNA序列相似性为97.9%,而氨基酸序列的相似性则达99.8%；中性检验表明ZmRCA1基因符合中性进化模型假设，没有发生纯化选择。
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| 关 键 词： | Rubisco活化酶(RCA) 单核苷酸多态性(SNP) InDel 连锁不平衡(LD) 单倍型 单倍型标签SNP |
| 收稿时间： | 2010-05-25 |
Analysis of Sequence Polymorphism of ZmRCA1 in Maize |
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| TAN Xian-Jie,SONG Yan-Chun,SHI Yun-Su,CHENG Wei-Dong,WU Zhi-Kai,WANG Tian-Yu,LI Yu.Analysis of Sequence Polymorphism of ZmRCA1 in Maize[J].Acta Agronomica Sinica,2011,37(1):58-66. |
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| Authors: | TAN Xian-Jie SONG Yan-Chun SHI Yun-Su CHENG Wei-Dong WU Zhi-Kai WANG Tian-Yu LI Yu |
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| Institution: | 1.School of Agronomy, Guangxi University, Nanning 530005, China;2.Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530227, China;3.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | Rubisco is a pivotal enzyme that initiates the first step of carbon fixation and photorespiration in plant photosynthesis. Rubisco accounts for up to half of the soluble protein in the leaves of plants. Nevertheless the catalytic rate of Rubisco iscomparatively low. Rubisco activity is regulated mainly by Rubisco activase, which serves as a molecular chaperone. By activating and regulating Rubisco, RCA potentially influences the efficiency of carbon assimilation in plants. Thereby, RCA has identified a possible target gene for improve production in crops breeding.To investigate polymorphism of ZmRCA1, sequened and analyzed the genomic sequences of ZmRCA1 from a minicore set of 95 maize inbreds lines. Totally 22 SNPs and 8 InDels were identified in a 1 680 bp sequence alignment. There were five SNPs and one InDel which generated amino acid sequence variation. A total of 27 haplotypes were identified with 13 polymorphic loci which the frequency was 0.1 or more. Approximately 90% haplotypes could be distinctly distinguished by six polymorphic loci. The ZmRCA1 gene was highly conserved, with the genic similarity of 97.9% and the amino acid sequence similarity of 99.8%. Neutrality tests showed that no purifying selection occurred in ZmRCA1. |
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| Keywords: | Rubisco activase (RCA) SNP InDel LD Haplotype htSNP |
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