Micropropagation of Anogeissus rotundifolia Blatt,and Hallb.-An Endemic and Rare Tree of the Thar Desert |
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| Abstract: | Abstract Multiple shoots and plantlets were developed in vitro from cotyledonary nodal segments of in vitro raised seedlings of Anogeissus rotundifolia (syn. A. sericea var. nummularia)-a rare and endemic tree species of the Thar Desert. About 15-20 shoots differentiated from a single cotyledonary node within four weeks on Mu-rashige and Skoog's (MS) basal medium containing 0.1 mg l?1 indole-3-acetic acid (IAA) + 2.0 mg l?1 6-benzylaminopurine (BAP) + additives (25 mg l?1 each of adenine sulphate, L-arginine, and citric acid, and 50 mg l?1 of ascorbic acid) at 26 ± 2°C temperature and 36 μmol m?2 s?1 photon flux density with a 12 h/day photoperiod. The shoots produced in vitro were further multiplied by subculturing on fresh medium. The original cotyledonary nodal segment was repeatedly transferred (5 to 6 times) onto fresh medium containing 1.0 mg l ?1 BAP + 0.1 mg I ?1 IAA + additives to yield fresh crops of multiple shoots. These shoots were rooted on half-strength MS medium supplemented with 0.1 mg l?1 indole-3-butyric acid (IBA). Plantlets were transferred to pots containing sand-dune soil and ver-miculite it the ratio of 4:1 (v/v) and hardened in a growth chamber for two weeks and finally transferred to a greenhouse. From a single cotyledonary node about 1500 plantlets could be developed within four months. The method developed is useful for mass multiplication and for the conservation of germplasm of Anogeissus rotundifolia. |
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| Keywords: | Wildfire population community |
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