| 云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析 |
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| 引用本文： | 和志娇,蔡红,陈海如,和根强,吴华英,唐丽明.云南泡桐丛枝病植原体核糖体蛋白基因片段序列分析[J].植物病理学报,2005,35(Z1):18-21. |
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| 作者姓名： | 和志娇 蔡红 陈海如 和根强 吴华英 唐丽明 |
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| 作者单位： | 1 云南农业大学 云南省植物病理重点实验室, 昆明 650201;2 云南省潞西市市农业局经济作物技术推广站, 芒市 678400 |
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| 基金项目： | 国家自然科学基金资助项目(30260064)
云南省自然科学基金资助项目(2000C0014Q) |
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| 摘 要： | 应用植原体核糖体蛋白基因通用引物对rpF1/rpR1,对采自云南省曲靖市的泡桐丛枝病植原体DNA (PaWB-QJ)进行PCR扩增,得到1.3 kb的特异片段,证明此病株中存在植原体。将此片段与pGEM-T Easy载体连接并转化大肠杆菌JM109感受态细胞,进行PCR鉴定、核糖体蛋白基因部分核苷酸序列测定及分析。结果表明,该株系(PaWB-QJ)核糖体蛋白基因片段长1 244 bp,包含rps19、rpl22和rps3基因。对PaWB-QJ株系的核糖体蛋白基因序列的同源性比较结果显示与16S rI-B亚组的翠菊黄化(Aster yellows,AY)、长春花黄化(Periwinkle yellows,PY)和泡桐丛枝德国株系(Paulownia witches'-broom,PaWB-German)的亲缘关系最近,达到99.0%以上,而与其它组中的株系明显低于97.0%,所以认为该植原体株系属于翠菊黄化组B亚组(16SrI-B)。
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| 关 键 词： | 泡桐 丛枝 核糖体蛋白基因序列 植原体 翠菊黄化 |
Sequencing of ribosomal protein gene of phytoplasma associated with Paulownia witches'-broom in Yunnan province |
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| HE Zhi-jiao,CAI Hong,CHEN Hai-ru,HE Gen-qiang,WU Hua-ying,TANG Li-ming.Sequencing of ribosomal protein gene of phytoplasma associated with Paulownia witches'-broom in Yunnan province[J].Acta Phytopathologica Sinica,2005,35(Z1):18-21. |
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| Authors: | HE Zhi-jiao CAI Hong CHEN Hai-ru HE Gen-qiang WU Hua-ying TANG Li-ming |
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| Institution: | 1 Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China;2 Station for Popularizing Economic Crops, Agricultural Bureau of Luxi, Mangshi 678400, China |
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| Abstract: | Universal primer (rpFl/rpRl) for ribosomal protein gene was used to detect phytoplasma of paulownia witches'-broom(PaWB-QJ) by polymerase chain reaction and DNA fragment of 1. 3 kb was amplified. The result indicated existence of phytoplasma associated with the diseased plant. The amplified fragment was liga-ted into the competent cell of E. coli JM109 strain. Cloned DNA fragments were verified by PCR and nucleo-tide sequence analysis. The result indicated that the ribosomal protein gene of PaWB-QJ strain consists of 1 244 base pairs, which contains entire rpsl9, rpl22 and rps3 genes. Phylogenetic analysis revealed that PaWB-QJ shared the highest homology (over 99.0% )with Aster yellows(AY) ,Periwinkle yellows(PY)and Paulownia witches'-broom(PaWB-German) in Aster yellows subgroup (16SrI-B). But it is obviously under 97.0% with other groups. So the results made it clear that this phytoplasma strain is one of the members of Aster yellows phytoplasma subgroup. |
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| Keywords: | paulownia witches'-broom ribosomal protein gene phytoplasma Aster yellows |
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