| 内生细菌及其组合对雷公藤悬浮细胞生长及生化特性的影响 |
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| 引用本文： | 王一惠,孙 睿,宋 萍,封 磊,吴承祯,洪 伟,宋 欢.内生细菌及其组合对雷公藤悬浮细胞生长及生化特性的影响[J].热带作物学报,2018,39(10):1947-1954. |
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| 作者姓名： | 王一惠 孙 睿 宋 萍 封 磊 吴承祯 洪 伟 宋 欢 |
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| 作者单位： | 1. 福建农林大学林学院，福建福州 350002；
2. 福建农林大学资源与环境学院，福建福州 350002；
3. 福建省高校森林生 态系统过程与经营重点实验室，福建福州 350002；
4. 武夷学院生态与资源工程学院，福建南平 354300 |
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| 摘 要： | 本研究将雷公藤内生细菌菌株 Pseudomonas chlororaphis LG6、Pantoea ananatis LY3、Bacillus cereus group LJ3、Bacillus cereus group LJ12 及其组合分别以活菌、灭活菌体粗提物和菌液的形式添加到悬浮细胞培养体系中，通 过检测悬浮细胞的重量、细胞活力以及培养液的 pH、电导率、可溶性糖和 NO3 ? 含量，分析了内生细菌对雷公藤悬浮细 胞生长状况的影响。结果显示，内生细菌活菌对悬浮细胞生长的影响较大，菌株 LY3、LJ3、LJ12、LY3+LJ12 及 LG6+LY3+LJ3+LJ12 活菌对悬浮细胞的鲜重具有促进作用，菌株 LY3 和大部分混合菌株的活菌降低了培养液的 pH，多 数内生菌株活菌提高了培养液的电导率，内生菌株活菌的添加使培养液的可溶性糖含量发生了较大变化，并明显提高 了培养液中的 NO3 ? 含量。灭活菌体粗提物和菌液的添加对细胞鲜重的增加多表现为抑制作用。
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| 关 键 词： | 内生细菌 雷公藤 悬浮细胞 生长 生化 |
Effects of Endophytic Bacteria and the Mixtures on Growth and Biochemical Properties of Suspension Cells of Tripterygium wilfordii |
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| WANG Yihui,SUN Rui,SONG Ping,FENG Lei,WU Chengzhen,HONG Wei,SONG Huan.Effects of Endophytic Bacteria and the Mixtures on Growth and Biochemical Properties of Suspension Cells of Tripterygium wilfordii[J].Chinese Journal of Tropical Crops,2018,39(10):1947-1954. |
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| Authors: | WANG Yihui SUN Rui SONG Ping FENG Lei WU Chengzhen HONG Wei SONG Huan |
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| Institution: | 1. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2. College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
3. Fujian Provincial Key Laboratory of Forest Ecosystem Processing and Management, Fuzhou, Fujian 350002, China; 4. College of Ecology and Resource Engineering, Wuyi University, Nanping, Fujian 354300, China |
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| Abstract: | In this study, endophytic bacterial strains Pseudomonas chlororaphis LG6, Pantoea ananatis LY3, Bacillus cereus group LJ3, Bacillus cereus group LJ12 isolated from Tripterygium wilfordii and their mixtures were made into living strains, crude extracts of dead thallus and bacterium solution, and then were added into the culture systems of plant suspension cells. The growth status of suspension cells after the addition of endophytic strains were analyzed by examining fresh weight and activity of suspension cells, pH value, electrical conductivity, soluble sugar and NO3 ? content of culture media. The results indicated that the living endophytic strains had a great effect on the growth of suspension cells. Living strains of LY3, LJ3, LJ12, LY3+LJ12 and LG6+LY3+LJ3+LJ12 promoted cell fresh weight compared to the control. The living strains of LY3 and majority of mixtures decreased pH values of the culture media. Compared to those of the control, the electrical conductivity of the culture media treated by adding most of the living strains increased. The living endophytic strains were also found to change soluble sugar content and increase NO3 ? content of the culture media compared to the control. Different from the living strains, the crude extracts of the dead thallus and bacterium solution disturbed the cell growth and decreased cell fresh weight compared to the control. |
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| Keywords: | endophytic bacteria Tripterygium wilfordii suspension cells growth biochemistry |
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