| 市售香蕉 15 种酚酸化合物含量的分析 |
| |
| 引用本文： | 李 涛,刘元靖,叶剑芝,杨春亮.市售香蕉 15 种酚酸化合物含量的分析[J].热带作物学报,2018,39(10):2047-2053. |
| |
| 作者姓名： | 李 涛 刘元靖 叶剑芝 杨春亮 |
| |
| 作者单位： | 中国热带农业科学院农产品加工研究所，广东湛江 524001 |
| |
| 摘 要： | 为了探明香蕉中酚酸化合物的种类及含量，本研究采用超高效液相色谱串联质谱（UPLC-MS/MS）法对 香蕉果肉样品进行分析，并对色谱、质谱条件进行优化。结果表明，香蕉富含多种酚酸化合物，且酚酸化合物主 要以游离酯型和结合型存在。含量较高的 2 种酚酸为异阿魏酸（112.54~370.34 μg/g）、阿魏酸（83.96~157.54 μg/g）。 酚酸含量由高至低依次为异阿魏酸>阿魏酸>对香豆酸>3,5-二羟基苯甲酸>芥子酸>香草酸>咖啡酸>对羟基苯甲 酸>鞣花酸>丁香酸>反式肉桂酸>水杨酸。方法验证结果表明该方法检出限低、线性范围宽、灵敏度高，定量结 果准确、可靠，适用于实际样品检测；香蕉样品酚酸组分与含量检测结果可为香蕉的营养成分和功能活性物质 评价提供参考。
|
| 关 键 词： | 香蕉 酚酸 超高效液相色谱串联质谱（UPLC-MS/MS）法 |
Analysis of 15 Phenolic Acids Compounds in the Bananas on Sale |
| |
| LI Tao,LIU Yuanjing,YE Jianzhi,YANG Chunliang.Analysis of 15 Phenolic Acids Compounds in the Bananas on Sale[J].Chinese Journal of Tropical Crops,2018,39(10):2047-2053. |
| |
| Authors: | LI Tao LIU Yuanjing YE Jianzhi YANG Chunliang |
| |
| Institution: | Agriculture Products Processing Research Institute, Chinese Academy of Tropical Agriculture Science, Zhanjiang, Guangdong 524001, |
| |
| Abstract: | In order to find out the type and content of phenolic acid compounds in bananas, the ultra performance Liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was employed to analyze banana samples and the experimental conditions of chromatography and mass spectrometry were optimized. The results showed that bananas were rich in various phenolic acid compounds,which were mainly presented in free-esterified and bound forms. Isoferric acid and ferulic acid were the two phenolic acids with higher content of 370.34 to 112.54 μg/g and 157.54 to 83.96 μg/g, respectively. Other phenolic acids were found in content order as follows: isoferulic acid, ferulic acid, p-coumaric acid, 3,5-dihydroxybenzoic, sinapic acid, vanillic acid, caffeic acid, p-hydroxybenzoic acid, ellagic acid, syringic acid, trans-cinnamic acid, salicylic acid. The developed method showed low detection limit, wide linear range and high sensitivity. The quantitative results were accurate, reliable and therefore suitable for the determination of real samples. The analysis results of phenolic acids components and content in samples provide reference for the evaluation of nutrients and functional and active substances in bananas. |
| |
| Keywords: | banana phenolic acid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
