| 水果及其制品中果胶含量的比色法测定条件优化 |
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| 引用本文： | 庞荣丽,张巧莲,郭琳琳,方金豹,谢汉忠,李君,罗静,吴丰魁.水果及其制品中果胶含量的比色法测定条件优化[J].果树学报,2012(2):302-307. |
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| 作者姓名： | 庞荣丽 张巧莲 郭琳琳 方金豹 谢汉忠 李君 罗静 吴丰魁 |
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| 作者单位： | 中国农业科学院郑州果树研究所/农业部果品及苗木质量监督检验测试中心(郑州);许昌职业技术学院园林园艺系 |
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| 基金项目： | 农业部2006年农业行业标准制修定项目(农财发[2006]64号) |
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| 摘 要： | 为研究水果及其制品中果胶测定方法,以半乳糖醛酸为标准物质,对硫酸-咔唑比色法测定水果及其制品中果胶含量的比色条件如吸收波长、咔唑-无水乙醇溶液用量、硫酸用量、显色温度、显色时间、显色后稳定时间等进行了优化和系统研究。结果表明,最佳显色条件为在1.0 mL滤液中,加入0.25 mL 0.1%咔唑-无水乙醇溶液,迅速加入5.0 mL硫酸,在85℃水浴中加热20 min,自来水浴中冷却,显色后1.5 h内在525 nm波长条件下测定。在此条件下,标准曲线线性范围广、相关性良好,精密度和准确度高,均符合相关标准要求。
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| 关 键 词： | 果胶 比色法 硫酸 咔唑 |
Study on the colorimetry determination conditions of pectin in fruits and derived products |
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| PANG Rong-li,ZHANG Qiao-lian,GUO Lin-lin,FANG Jin-bao,XIE Han-zhong,LI Jun,LUO Jing,WU Feng-kui.Study on the colorimetry determination conditions of pectin in fruits and derived products[J].Journal of Fruit Science,2012(2):302-307. |
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| Authors: | PANG Rong-li ZHANG Qiao-lian GUO Lin-lin FANG Jin-bao XIE Han-zhong LI Jun LUO Jing WU Feng-kui |
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| Institution: | 1(1Zhengzhou Fruit Research Institute,CAAS/ Monitor & Test Center of Fruit and Nursery Stock Quality,the Ministry of Agriculture,Zhengzhou,Henan 450009 China;2Department of Landscape & Horticulture,Xuchang Vocational Technical College,Xuchang,Henan 461000 China) |
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| Abstract: | In order to research the determination method of pectin content in fruits and derived products,coloring conditions of sulfuric acid-carbazole colorimetry including absorption wavelength,amount of carbazole-ethanol solution and sulfuric acid,temperature,reaction time,and stabilization time after coloring were optimized using galacturonic acid as standard substance.The results showed that the optimum coloring conditions were adding 0.25 mL 0.1% carbazole-ethanol solution and adding 5.0 mL sulfuric acid quickly to 1.0 mL filtrate,water bathing for 20 min at 85 ℃,cooling down quickly with running tape water bath,and measuring at 525 nm wavelength within 1.5 h after coloring.Under the conditions,it had wide linear range standard curve,good correlation,high precision and accuracy,and were in line with relevant standards. |
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| Keywords: | Pectin Colorimetry Sulfuric acid Carbazole |
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