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不同产地麦冬遗传多样性的SRAP分析
引用本文:马秀杰,王冠明,韩烈保.不同产地麦冬遗传多样性的SRAP分析[J].草业科学,2012,29(11):1686-1691.
作者姓名:马秀杰  王冠明  韩烈保
作者单位:北京林业大学草坪研究所暨森林培育与保护省部共建实验室,北京,100083;鸿铭控股有限公司,北京,100020;北京林业大学草坪研究所暨森林培育与保护省部共建实验室,北京100083 长江大学园艺园林学院,湖北荆州484025
基金项目:北京市教育委员会--北京市重点学科建设项目
摘    要:本研究使用SRAP分子标记技术对48份不同产地的麦冬进行遗传多样性分析,筛选出11对有效引物组合。11对引物组合共扩增出621条清晰、可辨认条带,平均每对引物扩增出56.45条带纹,多态性条带为621条,多态性位点百分率为100%,遗传相似系数(GS)范围为 0.058 4~0.936 7,平均为0.497 5,说明供试材料具有丰富的遗传多样性。对所有材料聚类分析发现,在遗传距离0.81处可将材料分为六大类群,部分来自同一地理位置的材料可聚为一类

关 键 词:沿阶草属  山麦科属  SRAP  遗传多样性

Genetic diversity in Lilyturf germplasm detected by SRAP markers
MA Xiu jie,WANG Guan ming,HAN Lie bao.Genetic diversity in Lilyturf germplasm detected by SRAP markers[J].Pratacultural Science,2012,29(11):1686-1691.
Authors:MA Xiu jie  WANG Guan ming  HAN Lie bao
Abstract:Genetic diversity of 48 Lilyturfs from different habitats was analyzed by using molecular marker SRAP. 11 effective primer combinations were filtered out and 621 bands with clearly identifiable section were amplified. Each pair of primers could amplify 56.45 bands and the number of polymorphism bands was 621. Moreover, the percentage of polymorphism loci was 100% and genetic similarity coefficient (GS) ranged from 0.058 4-0.936 7 with average value 0.497 5. These showed that the test materials were rich in genetic diversity. Cluster analysis of all materials indicated that the test materials could be divided into six groups at the genetic distance of 0.81 and some materials from the same geographical location could be classified as one group.
Keywords:Ophiopogon  Liriope  SRAP  genetic diversity
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