| ITS2序列作为DNA条形码在苍耳属中的应用 |
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| 引用本文： | 胡伟毅,赵晓燕.ITS2序列作为DNA条形码在苍耳属中的应用[J].安徽农业科学,2013(20):8472-8473,8504. |
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| 作者姓名： | 胡伟毅 赵晓燕 |
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| 作者单位： | 连云港出入境检验检疫局,江苏连云港,222042;江苏恒瑞医药股份有限公司,江苏连云港,222042 |
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| 基金项目： | 江苏出入境检验检疫局科技项目（2012KJ54）. |
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| 摘 要： | 目的]苍耳(属)(非中国种)植物为检疫性有害生物,该试验尝试以ITS2序列作为DNA条形码对从国外截获的7种苍耳属植物进行物种区分鉴定研究。方法]试验应用通用引物对其ITS2基因进行扩增,测序得到7种苍耳属植物的ITS2序列,利用MEGA 5.1软件得到的ITS2序列进行比对和分析,并构建系统树。结果]共发现变异位点242个,其中单突变位点12个。结论]ITS2序列能从基因位点层面对苍耳属植物进行区分鉴定。
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| 关 键 词： | ITS2序列 DNA条形码 苍耳属 |
The Application of ITS2 Sequence as DNA Barcode in Xanthium |
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| Institution: | HU Wei-yi et al (Lianyungang Entry-exit Inspection and Quarantine Bureau, Lianyungang, Jiangsu 222042) |
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| Abstract: | Objective] All the species in Xanthium （non-Chinese） are defined as quarantine pests. In this experiment, ITS2 sequence was used as DNA barcode to distinguish and indentify 7 species in Xanthium. Method] Universal primer was used to amplify ITS2 genes which were then sequenced. The ITS2 sequences were contrasted and analyzed by MEGA 5.1 software, and the phylogenetic tree was constructed. Result ] 242 variable sites and 12 singleton sites were discovered. Conclusion] The result shows that the species in Xanthium can be distinguished and indentified by the gene sites of ITS2 sequence. |
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| Keywords: | ITS2 sequence DNA barcode Xanthium |
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