| 纤维素酶提取工艺及酶学性质的研究 |
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| 引用本文： | 缪静,张术臻,程显好,冯志彬,王营营.纤维素酶提取工艺及酶学性质的研究[J].安徽农业科学,2008,36(19). |
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| 作者姓名： | 缪静 张术臻 程显好 冯志彬 王营营 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025;北京中棉紫光生物科技有限公司,北京,100044 |
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| 基金项目： | 鲁东大学校科研和教改项目 |
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| 摘 要： | 目的]探讨纤维素酶的最佳提取工艺和最佳反应条件。方法]以里氏木霉Rut C-30为发酵菌种,在30℃、摇床转速170 r/min条件下培养8 d,发酵生产纤维素酶,用盐析技术对粗酶液进行分离纯化,通过正交实验法探讨了纤维素酶的提取工艺条件。并以羧甲基纤维素钠酶活力为指标,对该酶的最佳反应条件和稳定性进行了研究。结果]纤维素酶的最佳提取条件是:提取时间为16 h、盐析饱和度为70%、pH值为4.8。纤维素酶的最佳反应条件是:pH值为4.8、温度为60℃。酶在pH 3.6~7.0时较稳定,在78℃保温30 min下的残留酶活为50%。结论]该研究为酶的工业化生产提供参考数据。
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| 关 键 词： | 里氏木霉 纤维素酶 提取 酶学性质 |
Study on the Extraction Technology and Enzymatic Properties of Cellulase |
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| MIAO Jing et al.Study on the Extraction Technology and Enzymatic Properties of Cellulase[J].Journal of Anhui Agricultural Sciences,2008,36(19). |
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| Authors: | MIAO Jing |
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| Abstract: | Objective] The aim of the study was to discuss the optimum extraction technology and reaction condition of cellulase.Method] With Trichaderma reesei Rut C-30 as fermentation strain,it was cultured at 30 ℃ and rotation speed of 170 r/min for 8 d to produce cellulase by fermentation.The crude enzyme solution was purified by salting-out technology.The technological condition of extracting cellulase was discussed through orthogonal experiment.And with the activity of CMC-Na as index,the optimum reaction condition and stability of the cellulase were studied.Result] The optimum extraction condition of cellulase was extraction time of 16 h,salting-out degree of 70% and pH value of 4.8.The optimum reaction condition of cellulase was pH value of 4.8 and temperature of 60 ℃.When pH value was 3.6-7.0,the cellulase was relatively stable,its residual enzyme activity was 50% after it was kept at 78 ℃ for 30 min.Conclusion] The study provided reference data for the industrial production of enzyme. |
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| Keywords: | Trichaderma reesei Cellulase Extraction Enzymatic properties |
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