| 红树内生细菌AmS2防治香蕉采后炭疽病的研究 |
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| 引用本文： | 欧雄常,柳 凤,何 红,昝丽梅,詹儒林.红树内生细菌AmS2防治香蕉采后炭疽病的研究[J].保鲜与加工,2013,13(5):15-19. |
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| 作者姓名： | 欧雄常 柳 凤 何 红 昝丽梅 詹儒林 |
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| 作者单位： | 中国热带农业科学院南亚热带作物研究所, 农业部热带果树生物学重点实验室;中国热带农业科学院南亚热带作物研究所, 农业部热带果树生物学重点实验室;广东海洋大学农学院;中国热带农业科学院南亚热带作物研究所, 农业部热带果树生物学重点实验室;中国热带农业科学院南亚热带作物研究所, 农业部热带果树生物学重点实验室 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项（1630062012008）；公益性行业（农业）科研专项（201203092-3） |
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| 摘 要： | 采用平板对峙结合生长速率法测定分析了红树内生细菌AmS2菌株及其甲醇粗提液对香蕉炭疽病菌的抑制活性,并对其甲醇粗提液的防病效果进行了研究。结果表明,红树内生细菌AmS2菌株及其甲醇粗提液对香蕉炭疽病菌的抑菌带宽分别为12.0 mm和11.5 mm;毒力测定结果表明,AmS2甲醇粗提液对香蕉炭疽病菌的菌丝生长和孢子萌发均有较强的抑制作用,EC50分别为1.007 6 mg/mL和1.902 8 mg/mL;AmS2菌株的甲醇粗提液对香蕉炭疽病具有较好的防治效果,且随着提取液浓度的加大,防治效果不断提高,当AmS2甲醇粗提液使用浓度为4.0 mg/mL时,对接种香蕉炭疽病菌10 d后的防治效果为79.83%。
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| 关 键 词： | 红树 内生细菌 香蕉 炭疽病 |
Biological Control to Banana Anthracnose by Endophytic Bacterial Strain AmS2 from Mangrove |
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| OU Xiong-chang,LIU Feng,HE Hong,ZAN Li-mei,ZHAN Ru-lin.Biological Control to Banana Anthracnose by Endophytic Bacterial Strain AmS2 from Mangrove[J].Storage & Process,2013,13(5):15-19. |
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| Authors: | OU Xiong-chang LIU Feng HE Hong ZAN Li-mei ZHAN Ru-lin |
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| Institution: | 1. South Asia Tropical Crop Research Institute, CATAS, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang 524091, China; 2. Agriculture Colleges, Guangdong Ocean University, Zhanjiang 524088, China) |
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| Abstract: | Using dual culture method and growth rate method, we evaluated the inhibitory activity of endophytic bacterial strain AmS2 from mangrove and its methanol crude extracts on Colletotrichum musae, and studied the control efficacy of methanol crude extract of AmS2 strain. The results showed that, the inhibition zones of strain AmS2 and its methanol crude extracts were 12.0 mm and 11.5 mm respectively. The results of toxicity determination showed that, the EC50 values of antifungal extract of AmS2 on mycelial growth and conidial germination of C. musae were 1.007 6 mg/mL and 1.902 8 mg/mL respectively. The banana anthracnose was controlled effectively by the methanol crude extracts, and with the increasing of the extract concentration, the control effect continuously improved. When the concentration of AmS2 methanol crude extracts was 4.0 mg/mL, the control effect was 79.83% in 10 d after treatment. |
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| Keywords: | mangrove endophytic bacteria banana anthracnose |
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