| 中国西北地区披碱草属植物所带内生真菌的培养特征 |
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| 引用本文： | 宋辉,南志标,田沛.中国西北地区披碱草属植物所带内生真菌的培养特征[J].草业学报,2015,24(9):89-95. |
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| 作者姓名： | 宋辉 南志标 田沛 |
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| 作者单位： | 草地农业生态系统国家重点实验室，兰州大学草地农业科技学院，甘肃 兰州 730020 |
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| 基金项目： | 国家“973”项目，中央高校基本科研业务费专项资金 |
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| 摘 要： | 内生真菌与植物所形成的共生体普遍存在于冷季禾草中,共生体有利于提高宿主植物的生物和非生物抗性。虽然目前研究者们已对禾草内生真菌做了大量的研究,但是尚未发现对分布于中国西北地区披碱草属植物无性世代Neotyphodium内生真菌培养特征的研究。本研究从中国西北地区分离出15株来自不同地理种群披碱草属植物无性世代Neotyphodium内生真菌。通过分离培养,测定了这15株无性世代Neotyphodium内生真菌的菌落生长速度,结果显示,这15株无性世代Neotyphodium内生真菌菌落的平均生长速度为(0.41~0.85)mm/d,且生长速度与海拔具有相关性。分布在海拔低于3000m的无性世代Neotyphodium内生真菌的菌落生长速度比生长于海拔高于3000m菌落的生长速度快。观察菌落发现,分布在海拔高于3000m的菌落正面特征较为一致,而分布在海拔低于3000m的菌落正面特征出现不同。另外,通过多基因联合建树发现,分布在海拔高于3000m的无性世代Neotyphodium内生真菌聚在一个分支,而分布在海拔低于3000m的无性世代Neotyphodium内生真菌呈现星状分布。由于目前尚未对中国西北地区披碱草属植物无性世代Neotyphodium内生真菌命名。因此,希望本研究结果能为该菌的命名提供理论依据。
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| 关 键 词： | 内生真菌 披碱草 菌落 多样性 系统发育 |
| 收稿时间： | 2014-10-09 |
Characteristic of asexual endophytes isolated from Elymus species in northwest China |
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| SONG Hui,NAN Zhi-Biao,TIAN Pei.Characteristic of asexual endophytes isolated from Elymus species in northwest China[J].Acta Prataculturae Sinica,2015,24(9):89-95. |
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| Authors: | SONG Hui NAN Zhi-Biao TIAN Pei |
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| Abstract: | Forage grasses (Poaceae)often harbour endophytes belonging to the Neotyphodium and Epichlo(Clavicipitaceae,Ascomycota)genera.Neotyphodium endophytes,the asexual state of Epichlospecies,helps to protect cool-season grasses against biotic and abiotic stresses including drought,disease,herbivory and para-sitism and enhance aboveground and belowground vegetative and reproductive growth.There is little informa-tion on colonial diversity of asexual endophytes isolated from Elymus species in northwest Chinese.In the present study,different geographic populations of Elymus species were tested using the aniline blue screening method;15 endophyte strains were isolated from endophyte positive plants.The average growth rate (on pota-to dextrose agar)of the isolated strains was 0.41-0.85 mm/day and was correlated with altitude.Endophyte strains isolated from regions below an altitude of 3000 m grew faster than strains isolated from regions above 3000 m.The colonial morphology of endophyte strains isolated from regions above 3000 m was similar whereas the colonial morphology of endophyte strains isolated from lower altitudes differed.In addition,the phyloge-netic trees showed that endophytes collected above 3000 m were clustered whereas endophyte strains from low-er altitudes demonstrated a star-like topology.The results will provide a theoretical basis for the identification of endophyte from northwest Chinese Elymus species. |
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| Keywords: | endophyte Elymus colony diversity phylogeny |
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