Lipase-catalyzed preparation of human milk fat substitutes from palm stearin in a solvent-free system |
| |
| Authors: | Zou Xiao-Qiang Huang Jian-Hua Jin Qing-Zhe Liu Yuan-Fa Song Zhi-Hua Wang Xing-Guo |
| |
| Institution: | School of Food Science and Technology, Jiangnan University, State Key Laboratory of Food Science and Technology, 1800 Lihu Road, Wuxi 214122, Jiangsu, People's Republic of China. |
| |
| Abstract: | Human milk fat substitutes (HMFSs) were synthesized by lipozyme RM IM-catalyzed acidolysis of chemically interesterified palm stearin (mp = 58 °C) with mixed FAs from rapeseed oil, sunflower oil, palm kernel oil, stearic acid, and myristic acid in a solvent-free system. Response surface methodology (RSM) was used to model and optimize the reactions, and the factors chosen were reaction time, temperature, substrate molar ratio, and enzyme load. The optimal conditions generated from the models were as follows: reaction time, 3.4 h; temperature, 57 °C; substrate molar ratio, 14.6 mol/mol; and enzyme load, 10.7 wt % (by the weight of total substrates). Under these conditions, the contents of palmitic acid (PA) and PA at sn-2 position (sn-2 PA) were 29.7 and 62.8%, respectively, and other observed FAs were all within the range of FAs of HMF. The product was evaluated by the cited model, and a high score (85.8) was obtained, which indicated a high degree of similarity of the product to HMF. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
