| 皱皮木瓜超氧化物歧化酶分离纯化研究 |
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| 引用本文： | 柳建平.皱皮木瓜超氧化物歧化酶分离纯化研究[J].安徽农业科学,2009,37(1):222-223. |
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| 作者姓名： | 柳建平 |
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| 作者单位： | 重庆师范大学生命科学学院,重庆,400047 |
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| 基金项目： | 重庆市扶贫办科技扶贫项目资助 |
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| 摘 要： | 目的]探讨皱皮木瓜超氧化物歧化酶（SOD）分离纯化工艺。方法]以重庆綦江皱皮木瓜为试验材料,采用硫酸铵分级沉淀、Se-vage法除杂蛋白、丙酮再次沉淀脱色除杂和Sephadex G-100凝胶层析,分离纯化皱皮木瓜SOD。采用Folin-酚法测定蛋白质含量,用改良的邻苯三酚自氧化法测定酶活性。结果]皱皮木瓜SOD在层析过程中得到较好的分离纯化。其纯化倍数是178.72倍,回收率是4.94%,比活力是303.82 U/mg蛋白。SDS-PAGE纯度鉴定结果表明,电泳图为一条谱带,表明分离纯化到达电泳纯的样品。结论]采用该分离纯化工艺得到比活力较高,达到电泳纯的皱皮木瓜SOD样品。
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| 关 键 词： | 皱皮木瓜 超氧化物歧化酶(SOD) 分离纯化 |
On the Separation and Purification of Superoxide Dismutase from Chaenomeles spiciosa Nakai |
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| LIU Jian-ping.On the Separation and Purification of Superoxide Dismutase from Chaenomeles spiciosa Nakai[J].Journal of Anhui Agricultural Sciences,2009,37(1):222-223. |
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| Authors: | LIU Jian-ping |
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| Institution: | LIU Jian-ping(College of Life Science,Chongqing Normal University,Chongqing 400047) |
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| Abstract: | Objective] This study aimed to discuss the separation and purification technology of Superoxide Dismutase(SOD)from C.spiciosa Nakai.Method] Using the C.spiciosa Nakai in Chongqing as experiment materials,highly purified samples of SOD were obtained by ammonium sulfate precipitation,Sevage methods,acetone precipitation and Sephadex G-100 gel chromatography.The protein content was measured through Folin-phenol method and the enzyme activity was determined by pyrogallol autoxidation.Result] SOD of C.spicios... |
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| Keywords: | Chaenomeles spiciosa Nakai Superoxide Dismutase Separation and purification |
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