| 鸡大肝和大脾病抗原在免疫器官的定位和分布 |
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| 引用本文： | 冉茂菊,徐福南,洪大蓉.鸡大肝和大脾病抗原在免疫器官的定位和分布[J].南京农业大学学报,2000,23(1):77-80. |
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| 作者姓名： | 冉茂菊 徐福南 洪大蓉 |
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| 作者单位： | 1. 南京农业大学动物医学院,南京,210095
2. 南京铁道医学院电镜室,南京210009 |
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| 基金项目： | 国家自然科学基金!(39870 5 76 ) |
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| 摘 要： | 用免疫金－银法（ＩＧＳＳ）和免疫胶体细胞化学方法，对人工感染的大肝和大脾（ＢＬＳ）鸡鸡免疫器官作ＢＬＳ抗原（ＢＬＳＡｇ）定位，分布及其动态研究。结果感染后第１，７，１９周，在脾脏，盲肠扁桃体，胸腺，法氏囊，红骨髓和哈德尔氏腺组织中均检查到ＢＬＳＡｇ，６主要分布在淋巴细胞，网状细胞，网状上皮细胞，巨噬细胞，单核细胞、浆细胞的胞浆、内质网，线粒体和一些空泡内。ＢＬＳＡｇ阳性淋巴细胞早期主要分布在胸腺和
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| 关 键 词： | 鸡 大肝和大脾病 抗原 定位 分布 免疫预防 |
| 修稿时间： | 1999－01－08 |
Location and distribution of BLS antigen in the immune organs of big liver and spleen(BLS)disease in broiler breeders |
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| Ran Maoju,Xu Funan,Hong Darong.Location and distribution of BLS antigen in the immune organs of big liver and spleen(BLS)disease in broiler breeders[J].Journal of Nanjing Agricultural University,2000,23(1):77-80. |
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| Authors: | Ran Maoju Xu Funan Hong Darong |
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| Abstract: | Location and the distribution of BLS antigen(BLSAg)were dynamically observed from the immune organs of broiler breeders experimentally inoculated with BLS disease agent by the methods of immuno gold silver staining(IGSS)and immuno colloidal gold cytochemistry.The BLSAg appeared in the spleen,the tonsillia cecalia and thymus,the bursa Fabricius,the red marrow and Harderian's gland at the lst week,7th week and 19th week post inoculation(P.I.).The BLSAg mainly located in the cytoplasm,endoplasmic reticulum,the mitochondria and some vacuole of the lymphocytes,reticulocytes and the epithelia reticular cell,the macrophages,the monocytes and plasma cells.The BLSAg positive lymphocytes mainly distributed in the thymus and the areas of the T lymphocytes in the periperal immune organs at the early stage,and they appeared extensively in the thymus,bursa Fabricius and the area of T and B lymnhocytes in the periperal immune organ at the 7th week P.I..The results indicated that the BLS virus located in the cytoplasm.This also showed that the humoral immunoresponse(HI)played an important role at the early stage,and that both of HI and the cell mediated immunoresponse may be decreased in varying degree at the 7th week. |
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| Keywords: | broiler breeder big liver and spleen disease antigen location distribution immuno gold silver staining immuno colloidal gold cytochemistry |
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