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东方百合叶片组织培养研究
引用本文:张延龙,徐 炎,王洁纯.东方百合叶片组织培养研究[J].西北农林科技大学学报(社会科学版),2004,32(1):47-50.
作者姓名:张延龙  徐 炎  王洁纯
作者单位:西北农林科技大学,园艺学院,陕西,杨凌,712100
基金项目:上海市农工商现代农业园区横向合作项目(141107)
摘    要:对东方百合杂交系(Orientalhybrids)中西西(Sissi)品种的叶片、叶柄进行了离体培养研究。结果表明,诱导西西叶片、叶柄分化出小鳞茎的培养基为S4(MS+6-BA2.0mg/L+NAA0.2mg/L),诱导率分别为22.2%,11.1%。S4培养基诱导的小鳞茎粗壮,再分化能力强,诱导率相对较高,宜采用MS+6-BA2.0mg/L+NAA0.2mg/L培养基作为继代培养基;生根培养基为1/2MS+IBA0.3mg/L。

关 键 词:东方百合  组织培养  叶片  叶柄  小鳞茎
文章编号:1671-9387(2004)01-0047-04
收稿时间:2003/7/15 0:00:00
修稿时间:2003年7月15日

Tissue culture of oriental lily hybrids
ZHANG Yan-long,XU Yan,WANG Jie-chun,YANG Zhi-juan.Tissue culture of oriental lily hybrids[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(1):47-50.
Authors:ZHANG Yan-long  XU Yan  WANG Jie-chun  YANG Zhi-juan
Institution:(College of Horticulture,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China)
Abstract:The leaf,petiole of the oriental lily hybrids,Sissi was in vitro cultured.The optimal medium for the leaf,petiole of Sissi was S4 medium (MS+6-BA (2.0 mg/L)+NAA 0.2 mg/L),and the inducing rate of bublets was 22.2%,11.1% respectively.The bulblets differentiated on the S4 (MS+6-BA (2.0 mg/L)+NAA 0.2 mg/L) medium was better,and the inducing rate was higher.The subculture medium was the S4 (MS+6-BA (2.0 mg/L)+NAA 0.2 mg/L) medium.The rooting medium was 1/2 MS+IBA (0.3 mg/L).
Keywords:oriental lily hybrids  tissue culture  leaf  petiole  bublet
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