Somaclonal variation in sugarcane through tissue culture and evaluation for quantitative and quality traits |
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| Authors: | S Rajeswari S Thirugnanakumar A Anandan and M Krishnamurthi |
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| Institution: | (1) E.I.D. Parry (India) Ltd.,, Pugalur Sugar Factory, Pugalur, Karur, Tamil Nadu, India;(2) Department of Agricultural Botany, Faculty of Agriculture, Annamalai University, Annamalainagar, Cuddalore, Tamil Nadu, India |
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| Abstract: | Eight genotypes of Saccharum officinarum were crossed with Saccharum spontaneum and 14 genotypes of S. officinarum were crossed with Erianthus arundinaceus. A total of 39 hybrids were evolved. These 39 hybrids were raised in the field and used as donor clones for in vitro culture
studies. Plantlets were regenerated from 1-month-old callus. The grown up plants were transplanted to well prepared field,
to study the variations generated for the biometric as well as for biochemical characters. There were significant differences
between the donor clones and their sub clones for all the character of interest. The somatic segregation was gradual and wider,
showing a range of divergence from the mean towards the end of the scale. Fifty-one sub clones were selected with commercial
potential which have 13% fibre, 200 cm stalk length, 10 cm internode length and pure obtainable cane sugar per cent of 10. |
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| Keywords: | Erianthus arundinaceus Saccharum officinarum S spontaneum Somaclonal variation |
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