| 马铃薯晚疫病菌SSR扩增产物的检测方法比较 |
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| 引用本文： | 孟然然,郝大海,王兆富,李周,胡艳梅,李灿辉.马铃薯晚疫病菌SSR扩增产物的检测方法比较[J].安徽农学通报,2012,18(13):23-24,71. |
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| 作者姓名： | 孟然然 郝大海 王兆富 李周 胡艳梅 李灿辉 |
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| 作者单位： | 1. 云南师范大学薯类作物研究所,云南昆明,650500
2. 普洱市农业科学研究所,云南普洱,665000 |
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| 基金项目： | 农业部公益性(农业)行业科研专项3-20;云南省科技厅2008CA027、2008PY053和2009BB01资助 |
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| 摘 要： | 对39份马铃薯晚疫病菌菌株的SSR扩增产物进行聚丙烯酰胺凝胶电泳后,分别用银染、溴化乙锭(EB)染色并统计清晰特征条带。实验结果表明银染分辨率高,但检测到较多的杂带,EB染色的灵敏度虽比银染差,仍能获得高质量的目的条带;理论计算的SSR目的条带的质量范围是147.5～226.7 ng,都超过了2种染色方法需要的最小理论质量。实验结果和理论分析同时表明2种染色方法皆可用于马铃薯晚疫病群体多样性的研究。
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| 关 键 词： | 马铃薯晚疫病 SSR 银染 EB染 |
Comparison of Methods for Detecting SSR amplification products in Phytophthora infestans |
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| Institution: | Meng Ranran et al. ( 1 Root and Tuber Crops Research Institute, Yunnan Normal University, Kunming 650500, China) |
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| Abstract: | After polyacrylamide gel electrophoresis using SSR products of 39 strains isolated, silver staining and ethidium bromide （EB） staining were used to band statistic, the results show that the silver staining method lead to high resolution, but also detected more minor band, EB staining method was less sensitivity than silver staining. However,it can detect more qualified target band. In theory, calculation of SSR band quality were range from 147.5 - 226.7 ng, representing higher col- oration quality requirements then two staining methods. The experimental results and theoretical analysis also appear that the two staining methods can be used for p. infestans population study. |
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| Keywords: | Phytophthora infestans SSR Silver Staining Ethidium bromide staining |
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