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金桂的组织培养与快速繁殖
引用本文:吕志鹏.金桂的组织培养与快速繁殖[J].安徽农学通报,2012,18(8):27-27,165.
作者姓名:吕志鹏
作者单位:福建林业科技试验中心,福建南靖,363600
摘    要:以金桂茎段为试验材料,采用正交试验设计方案,探讨大量元素和不同生长调节物质对金桂诱导、增殖以及生根过程中的影响。结果表明:金桂的诱导培养基以MS+6-BA2.5mg/L+NAA0.5mg/L较为适宜;增殖培养基以B5+6-BA3.0mg/L+NAA0.4mg/L为最佳;生根培养基是:改良MS+IBA0.5mg/L+NAA0.02mg/L,生根率可达85%以上。

关 键 词:桂花  茎段  快速繁殖

Tissue Culture and Rapid Propagation of jin gui
Lv Zhipeng.Tissue Culture and Rapid Propagation of jin gui[J].Auhui Agricultural Science Bulletin,2012,18(8):27-27,165.
Authors:Lv Zhipeng
Institution:Lv Zhipeng (The Experiment Center of Forestry Science and Technique of Fujian Province,Nanjing 363600,China)
Abstract:In order to get the optimal medium to culture Osmanthus fragrans.,semi-ligneous stem sections of a dominant tree in a mature forest were used as explants and orthogonal with the compound of three elements(macroelements,BA and NAA) with three levels was adopted in this study.The results showed that the optimum medium for regeneration bud culture were MS + 6-BA2.5mg/L +NAA0.5mg/L,the medium for sub-propagation were B5 + 6-BA3.0mg/L +NAA0.4mg/L,improved MS + IBA0.5mg/L+ NAA0.02mg/L was successful rooting.It was that ratio of the growth rooting reached 85%.
Keywords:Osmanthus fragrans  Stem section  Rapid propagation
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