| 犬细小病毒病的PCR临床诊断研究 |
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| 引用本文： | 姚大伟,侯加法,沈向真,杨德吉.犬细小病毒病的PCR临床诊断研究[J].安徽农学通报,2007,13(8):73-75. |
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| 作者姓名： | 姚大伟 侯加法 沈向真 杨德吉 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095 |
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| 摘 要： | 建立犬细小病毒聚合酶链式反应(PCR)检测方法,并应用于临床诊断.根据犬细小病毒基因保守序列设计一对特异性引物,扩增目的片段大小为448bp.用此引物扩增疫苗中的犬细小病毒和37份病料中的犬细小病毒,并与胶体金诊断试剂盒相对比.实验结果显示:该方法能从疫苗中和临床病料中扩增出犬细小病毒目的基因片段,与胶体金诊断试剂盒相比能克服免疫血清学诊断中的非特异性反应,具有快速、准确、灵敏度高的优点,完全适用于犬细小病毒病的临床诊断.
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| 关 键 词： | 聚合酶链式反应 犬细小病毒 临床诊断 |
| 文章编号： | 1007-7731（2007）08-73-03 |
| 收稿时间： | 2007-03-20 |
| 修稿时间： | 2007年3月20日 |
Study on Clinical Diagnosis for Canine parvovirus disease by PCR |
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| Yao Da-wei,Hou Jia-fa,Shen Xiang-zhen,Yang De-ji.Study on Clinical Diagnosis for Canine parvovirus disease by PCR[J].Auhui Agricultural Science Bulletin,2007,13(8):73-75. |
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| Authors: | Yao Da-wei Hou Jia-fa Shen Xiang-zhen Yang De-ji |
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| Institution: | College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Development and Application of Polymerase Chain Reaction test methods for Canine parvovirus.A pair of specific primer was designed according to the consensus sequence of canine parvovirus.A 448bp gene product was amplified by PCR.Vaccine and 37 clinical pathological samples were detected for canine parvovirus by PCR.It showed that this method can overcome the nonspecific reaction of serological diagnostics,compared with immunochromatographic assay,and it has many strongpoints such as speediness,nicety and sensitivity.This method completely fit for clinical diagnosis of canine parvovirus disease. |
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| Keywords: | polymerase chain reaction canine parvovirus diagnosis |
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