Genetic analysis of an attenuated <Emphasis Type="Italic">Papaya ringspot virus</Emphasis> strain applied for cross-protection |
| |
| Authors: | Chu-Hui Chiang Chun-Yee Lee Ching-Hsien Wang Fuh-Jyh Jan Shih-Shun Lin Tsung-Chi Chen Joseph A J Raja Shyi-Dong Yeh |
| |
| Institution: | (1) Department of Molecular Biotechnology, Da Yeh University, Chang Hwa, Taiwan;(2) Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan |
| |
| Abstract: | Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection,
was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between
HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in P1 and HC-Pro genes caused attenuated infection on papaya without conspicuous
symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal
region of P1 and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on
papaya. We conclude that both P1 and HC-Pro bear important pathogenicity determinants for the infection on the systemic host
papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce
hypersensitive reaction on C. quinoa. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
