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广西黑皮冬瓜疫病的病原菌鉴定及其生物学特性
引用本文:李卫民,晏卫红,黄思良,陆少峰,陈景成,岑贞陆,付岗.广西黑皮冬瓜疫病的病原菌鉴定及其生物学特性[J].植物病理学报,2007,37(3):333-336.
作者姓名:李卫民  晏卫红  黄思良  陆少峰  陈景成  岑贞陆  付岗
作者单位:1 广西农业科学院, 南宁 530007;2 长江大学农学院, 荆州 434025;3 广西玉林市植保站, 玉林 537000
摘    要: Isolate PD1 of Phytophthora was isolated from diseased black pericarp wax gourd in Guangxi in 2005. The morphological and biological characters of the isolate were examined, and its sequence of ribosomal DNA-ITS was analyzed. The range of growth temperature of the isolate was 10-37℃ and the optimal one was 31℃. The pathogen was sensitive to 10 mg/kg of malachite green, and its ability for starch utilization was weak. A wide host range of the pathogen was confirmed by artificial inoculation. The sizes of sporangia were (37.5-71.7) μm×(20.8-47.5) μm (av. 55.0 μm×30.4 μm). Microcycle conidiation of the pathogen was observed. The pathogen was identified as Phytophthora drechsleri Tucker based on its morphological and biological characters as well as ribosomal DNA-ITS sequence.

关 键 词:广西壮族自治区  病原菌鉴定  黑皮冬瓜  生物学特性  疫病  大面积种植  病原菌种类  ITS序列
文章编号:0412-0914(2007)03-0333-04
收稿时间:2006-07-13
修稿时间:2006-07-132006-11-14

Identification and biological characters of the causal organism of phytophthora blight of black pericarp wax gourd in Guangxi
LI Wei-min,YAN Wei-hong,HUANG Si-liang,LU Shao-feng,CHEN Jing-cheng,CEN Zhen-lu,FU Gang.Identification and biological characters of the causal organism of phytophthora blight of black pericarp wax gourd in Guangxi[J].Acta Phytopathologica Sinica,2007,37(3):333-336.
Authors:LI Wei-min  YAN Wei-hong  HUANG Si-liang  LU Shao-feng  CHEN Jing-cheng  CEN Zhen-lu  FU Gang
Institution:1 Guangxi Academy of Agricultural Sciences, Nanning 530007, China;2 College of Agriculture, Yangtze University, Jingzhou 434025, China;3 Guangxi Yulin Plant Protection Station, Yulin 537000, China
Abstract:Isolate PD1 of Phytophthora was isolated from diseased black pericarp wax gourd in Guangxi in 2005. The morphological and biological characters of the isolate were examined, and its sequence of ribosomal DNA-ITS was analyzed. The range of growth temperature of the isolate was 10-37℃ and the optimal one was 31℃. The pathogen was sensitive to 10 mg/kg of malachite green, and its ability for starch utilization was weak. A wide host range of the pathogen was confirmed by artificial inoculation. The sizes of sporangia were (37.5-71.7) μm×(20.8-47.5) μm (av. 55.0 μm×30.4 μm). Microcycle conidiation of the pathogen was observed. The pathogen was identified as Phytophthora drechsleri Tucker based on its morphological and biological characters as well as ribosomal DNA-ITS sequence.
Keywords:black pericarp wax gourd  Phytophthora drechsleri  identification  biological character  ribosomal DNA-ITS
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