| 紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应 |
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| 引用本文： | 王 洲,杨超英,薛正莲.紫外辐照对产α-ALDC枯草芽孢杆菌的诱变效应[J].中国农学通报,2007,23(4):95-95. |
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| 作者姓名： | 王 洲 杨超英 薛正莲 |
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| 作者单位： | 安徽工程科技学院生物化学工程系,芜湖,241000 |
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| 基金项目： | 安徽省高校青年教师科研项目 |
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| 摘 要： | 采用紫外诱变(波长260nm，功率15w)对一株产α-乙酰乳酸脱羧酶的枯草芽孢杆菌（Bacillus subtilis）BS059菌株进行诱变处理。结果表明，在紫外辐照时间为50s时，诱变效果较好。从正突变菌株中反复筛选，得到两株产酶量较高的菌株BS059-15和BS059-22，比原出发菌株的酶活分别提高了107.62%和162.25%。经过连续传代试验，证明其遗传性状稳定，为可遗传变异。
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| 关 键 词： | 白藜芦醇 白藜芦醇 次生代谢产物 |
| 修稿时间： | 2007-01-222007-02-06 |
The Mutation Effect of UV Irradiation on a Strain Bacillus subtilis Producing α-acetolactate decarboxylase |
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| Wang Zhou,Yang Chaoying,Xue Zhenglian.The Mutation Effect of UV Irradiation on a Strain Bacillus subtilis Producing α-acetolactate decarboxylase[J].Chinese Agricultural Science Bulletin,2007,23(4):95-95. |
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| Authors: | Wang Zhou Yang Chaoying Xue Zhenglian |
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| Institution: | （Department of Biochemistry, Anhui University of Technology and Science, Wu’hu 241000） |
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| Abstract: | A study was made on Bacillus subtilis is strain BS059 with the production of α- acetolactate decarboxylase by the technology of UV irradiation (λ=260nm，W=15w). The result showed that optimal mutation effect was at the irradiation time of 50 seconds. After several screening tests, two mutant strain was named BS059-15 and BS059-22 whose enzyme activities was 107.62% and 162.25% higher than the original strain BS059 respectively. Then it was found that the yield of α-ALDC by the strain BS059-15 and BS059-22 were stable through successive transfer of culture. |
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| Keywords: | UV irradiationzz α- acetolactate decarboxylasezz Bacillus subtiliszz |
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