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| 温度和金属离子对干扰素诱导剂 聚肌胞配对的影响 | |
| 作者姓名： | 毛明光 王印庚 尹立杰 张家松 刘宗柱 |
| 作者单位： | 1. 青岛农业大学,生命科学学院,266109;中国水产科学研究院黄海水产研究所,青岛,266071 2. 中国水产科学研究院黄海水产研究所,青岛,266071 3. 青岛汉河动植物药业有限公司,266103 4. 青岛农业大学,生命科学学院,266109 |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家科技部科技支撑计划项目 |
| 摘 要： | 为提高聚肌胞（Polyinosinic-polycytidylic acid，polyI：C）的配对效果，本文研究了温度和金属离子对聚肌胞配对的影响。实验结果显示，在45℃条件下合成的聚肌胞减色效应最强，说明在该温度下聚肌苷酸（Polyinosinic acid，PI）和聚胞苷酸（Polycytidylic acid，PC）配对最紧密；Na^＋、Ca^2＋、Mg^2＋、Mn^2＋和Zn^2＋能够增强聚肌胞双链结构的紧密性，当Na^＋浓度增加到0．085mol／L后，聚肌胞的配对接近饱和；Ca^2＋、Mg^2＋、Mn^2＋和Zn^2＋对聚肌胞结构的保护效果基本一致，而Cu^2＋对聚肌胞结构具有明显破坏作用。建议采用45℃合成聚肌胞并添加适当浓度的Na^＋和Ca^2＋等增强其结构的紧密性，以避免机体内高活性RNA酶的降解而失去药效。 |
| 关 键 词： | 干扰素诱导剂 聚肌胞 配对 温度 金属离子 |
| 收稿时间： | 2008/3/15 0:00:00 |
| 修稿时间： | 2008/5/16 0:00:00 |
Effects of temperature and metallic cations on the combination of interferon inducer polyinosinic polycytidylic acid (polyI:C) |
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| Authors: | MAO Ming-guang WANG Yin-geng YIN Li-jie ZHANG Jia-song LIU Zong-zhu |
| Institution: | MAO Ming-guang WANG Yin-geng YIN Li-jie ZHANG Jia-song LIU Zong-zhu (1. Qingdao Aqrieultural University,266109) (2 Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071;2. Qingdao Hanhe Animal ＆ Plant Pharmaceutical Co., LTD,266102) |
| Abstract: | Effects of temperature and metallic cations on the synthesis of polyinosinicpolycytidylic acid were tested to improve its structure. The results showed significant hypochromic effects of polyI.C formed at 45 ℃, which indicated that the best combination of polyI.C were achieved at this temperature, while the metallic cations including Na^＋, Ca^2＋, Mg^2＋, Mn^2＋ and Zn^2＋ could enhance the polyI：C combination. 0. 085mol/L PolyI.C combination achieved saturation when Na^＋ concentration was, and the divalent cations including Ca^2＋、 Mg^2＋、 Mn^2＋ and Zn^2＋ played positive effect on the combination. However, Cu^2＋ tend to destroy the doublestranded structure. It is suggested that polyI：C should be made at 45℃ and suitable concentration of Na^＋ and Ca^2＋ should be added to make its structure more tight in order to avoid RNase＇ s digesting. This result is useful for synthesizing polyI：C at a high quality. |
| Keywords: | Interferon inducer Polyinosinic-polycytidylic acid Synthesis Temperature Metallic cations |
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