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一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位
引用本文:李超,林冬枝,董彦君,叶胜海,张小明.一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位[J].中国水稻科学,2010,24(3):223-227.
作者姓名:李超  林冬枝  董彦君  叶胜海  张小明
作者单位:1. 上海师范大学,生命与环境科学学院,植物遗传与功能基因研究室,上海,200234
2. 浙江省农业科学院,作物与核技术利用研究所,浙江,杭州,310021
基金项目:上海市科学技术委员会资助项目(08PJ14085,075405117,09DJ1400505,0939191200); 国家自然科学基金资助项目(30971552); 国家航天育种工程资助项目(2006HT100013)
摘    要:通过对粳稻品种嘉花1号60Coγ射线诱变,从M2中筛选出一株低温敏感型白色条斑叶突变体(tws)。它在低温(20℃,24℃)条件下培养时,苗期第3和第4叶表现出白色条斑,而第5叶开始转为正常。低温条件下该突变体白斑叶片叶绿素含量明显下降。该突变体白色条斑叶性状具有温敏感性,且与叶龄相关。遗传分析表明,该突变性状受1对隐性核基因控制,定名为tws(thermo-sensitive white stripe-leaf)基因。以tws突变体与籼稻9311杂交的F2分离群体作为定位群体,利用SSR标记将该基因定位在第4染色体MM3907和MM3928之间,其物理距离约为86kb。

关 键 词:水稻  温度敏感性  白色条斑叶
收稿时间:1900-01-01;

Genetic Analysis and Mapping of a Thermo-Sensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa)
LI Chao,LIN Dong-zhi,DONG Yan-jun,YE Sheng-hai,ZHANG Xiao-ming.Genetic Analysis and Mapping of a Thermo-Sensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa)[J].Chinese Journal of Rice Science,2010,24(3):223-227.
Authors:LI Chao  LIN Dong-zhi  DONG Yan-jun  YE Sheng-hai  ZHANG Xiao-ming
Institution:Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn
Abstract:A thermo sensitive white stripe leaf mutant (tws) was selected from the M2 progeny of a japonica rice variety, Jiahua 1, treated with 60Co γ radiation. In comparison with the wild type parent (Jiahua 1), the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn a normal green phenotype on the 5th leaf when grown at low temperatures (20℃, 24℃). And also the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermo sensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws (thermo sensitive white stripe leaf). In addition, by using SSR markers and an F2 segregating population derived from the cross between the tws mutant and 9311, the tws gene was mapped between MM3907 and MM3928 with a physical distance of 86 kb on chromosome 4.
Keywords:rice(Oryza sativa)  thermo-sensitivity  white stripe-leaf  gene mapping  
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