<Emphasis Type="Italic">Pseudomonas</Emphasis> sp. LSW25R,antagonistic to plant pathogens,promoted plant growth,and reduced blossom-end rot of tomato fruits in a hydroponic system |
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| Authors: | Sang-Woo Lee Il-Pyung Ahn Sang-Yeon Sim Sue-Yeon Lee Myeon-Whoon Seo Soonok Kim Sook-Young Park Yong-Hwan Lee Seogchan Kang |
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| Institution: | (1) Gyeonggi-Do Agricultural Research and Extension Services, Hwaseong, 445-300, South Korea;(2) National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, 441-707, South Korea;(3) Department of Agricultural Biotechnology and Center for Fungal Genetic Resource, Seoul National University, Seoul, 151-921, South Korea;(4) Department of Plant Pathology, The Pennsylvania State University, University Park, 16802, USA |
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| Abstract: | A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant
mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited
mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural
aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots
of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants
at 108 cfu·ml−1, and increased the plant growth of eggplants and peppers at 104 cfu·ml−1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover,
densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9.3 × 103 cfu·g−1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard
nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system,
LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity
and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake. |
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