| 绞股蓝组培苗添加水杨酸后总黄酮含量及苯丙氨酸解氨酶活性研究 |
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| 引用本文： | 孙蓉蓉,肖娅萍,成晓霞.绞股蓝组培苗添加水杨酸后总黄酮含量及苯丙氨酸解氨酶活性研究[J].安徽农学通报,2008,14(9):33-34. |
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| 作者姓名： | 孙蓉蓉 肖娅萍 成晓霞 |
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| 作者单位： | 教育部药用植物资源及天然药物化学重点实验室,陕西西安,710062;教育部药用植物资源及天然药物化学重点实验室,陕西西安,710062;陕西师范大学生命科学学院,陕西西安,710062 |
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| 摘 要： | 采用索氏提取法提取黄酮，可见分光光度法测定样品中总黄酮含量和PAL酶活性。结果表明：在50—150μmol／L浓度范围内附加水杨酸可提高绞股蓝组培苗的总黄酮含量和PAL酶活性。
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| 关 键 词： | 绞股蓝 总黄酮 PAL 水杨酸 |
| 文章编号： | 1007-7731(2008)09-33-02 |
| 修稿时间： | 2008年2月3日 |
Contents of Total Flavonoids and Activity of PAL in Gynostemma after Adding Licylic Acid |
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| Sun Rongrong,Xiao Yaping,Cheng Xiaoxia.Contents of Total Flavonoids and Activity of PAL in Gynostemma after Adding Licylic Acid[J].Auhui Agricultural Science Bulletin,2008,14(9):33-34. |
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| Authors: | Sun Rongrong Xiao Yaping Cheng Xiaoxia |
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| Institution: | Sun Rongrong, Xiao Yaping, Cheng Xiaoxia ( 1. Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Xihn 710062, Shanxi, China ; 2. Life Science College, Shanxi Normal University, Xihn 710062, Shanxi, China) |
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| Abstract: | Objective： Determine the total flavonoids and activity of PAL after adding SAlicylic acid. Methods： The total flavonoids were extraceted by Soxhlet extraction and determined by spectrophotometry. The activity of PAL also determined by spectrophotometry. Results： SA in the range of 50μmol/L - 150μmol/L can enhance the total ilavonoids contents and activity of PAL in culture Gynostemma. Conclusion ： Adding slicylic acid can apply in enhancing the total flavonoids contents in culture Gynostemma. |
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| Keywords: | PAL |
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