Analysis of Potassium Uptake by Rice Roots Treated with Aluminum Using a Positron Emitting Nuclide, 38K |
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| Authors: | Keitaro Tanoi Junko Hojo Kazutoshi Suzuki Yoshitake Hayashi Hiroki Nishiyama Tomoko M Nakanishi |
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| Institution: | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113–8657 Japan;Division of Advanced Technology for Medical Imaging, National Institute of Radiological Sciences, Inage-ku, Chiba, 263–8555 Japan |
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| Abstract: | We investigate the effect of Al on K+ uptake by rice roots. Potassium-38 (38K), a positron emitting nuclide (the half-life: 7.61 min), was used to trace K+ behavior. When a rice root was treated with 10μM Al for 24 h, the uptake of 38K in the root was increased in the range of 1 to 2 cm from the root tip compared with that of the control sample. Because the root continued to grow without showing any damage of plasma membrane during the Al treatment, it was suggested that the 38K uptake was not occurred through diffusion into the cells. The uptake of 38K in both treatments, with/without Al, was decreased by VO43- (inhibitor of H+-ATPase on plasma membrane) and DNP (H+ ionophore) treatment, which suggested that the K+ uptake was performed through an active transport, such as H+:K+ transport or H+ gradient promoted by an Al treatment. |
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| Keywords: | aluminum K-38 positron emitting nuclide potassium uptake proton pump rice |
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