| 反相高效液相色谱法测定金银花中绿原酸的含量 |
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| 引用本文： | 方传代.反相高效液相色谱法测定金银花中绿原酸的含量[J].安徽农业科学,2007,35(24):7396-7397. |
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| 作者姓名： | 方传代 |
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| 作者单位： | 宜春学院物理科学与工程技术学院,江西宜春,336000 |
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| 摘 要： | 为了研究金银花质量控制的有效手段。通过建立反相高效液相色谱法测定了金银花中绿原酸的含量。测定条件为:色谱柱为Nucleosil C18柱(4.6×250 mm,5μm),流动相为甲醇-0.4%磷酸(体积比为25∶75),流速1.0 ml/min,光电二极管阵列检测器,检测波长327 nm,柱温25℃。绿原酸在0.64~6.40μg范围内其进样量与峰面积呈良好的线性关系(r=0.999 6),平均回收率为99.3%,相对标准偏差(RSD)为0.8%。以6种不同浓度(0%~95%)的乙醇溶液为提取剂,对绿原酸的提取率进行考察的结果表明,30%乙醇溶液的绿原酸提取率最高,且杂质峰的数量明显减小。该方法具有简便、快速,回收率、精密度高的优点,可作为金银花质量控制的有效手段。
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| 关 键 词： | 金银花 绿原酸 含量 高效液相色谱法 光电二极管检测器 |
| 文章编号： | 0517-6611（2007）24-07396-02 |
| 修稿时间： | 2007-04-20 |
Determination of the chlorogenic Acid in Flos Lonicerae with HPLC |
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| FANG Chuan-dai.Determination of the chlorogenic Acid in Flos Lonicerae with HPLC[J].Journal of Anhui Agricultural Sciences,2007,35(24):7396-7397. |
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| Authors: | FANG Chuan-dai |
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| Institution: | Physical Science and Technology College, Yichun University, Yichun, Jiangxi 336000 |
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| Abstract: | The high performance liquid chromatographic method was developed for the determination of chlorogenic acid in Flos Lonicerae.The chromatographic separation was carried out in a Nucleosil C18 column(4.6×250 mm,5 μm) with a mobile phase consisting of methanol-0.4 %H3PO4(25∶75,V/V) mixture.The detection wavelength of photodiode array detector was set at 327 nm and the column temperature was set at 25 ℃.The calibration curve was linear in the range of 0.64～6.40 μg for chlorogenic acid(r=0.999 6).The average recovery of chlorogenic acid was 99.3 % with a relative standard deviation(RSD)of 0.8 %.The method was simple,reliable and suitable for quality evaluation and control of Flos Lonicerae. |
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| Keywords: | Flos Lonicerae Chlorogenic acid Content HPLC Photodiode array detector |
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