| 白灵侧耳菌株间的RAPD分析 |
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| 引用本文： | 胡润芳,薛珠政,唐永晖.白灵侧耳菌株间的RAPD分析[J].福建农业学报,2006,21(4):346-349. |
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| 作者姓名： | 胡润芳 薛珠政 唐永晖 |
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| 作者单位： | 1. 福建省农业科学院作物研究所,福建,福州,350013
2. 福建省连江县种子公司,福建,连江,350500 |
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| 摘 要： | 采用CTAB法提取白灵侧耳菌丝基因组DNA,利用RAPD技术分析13个白灵侧耳菌株间的亲缘关系。结果表明,从80个随机引物筛选出11个引物,共扩增出66条带,其中多态条带50条,多态性程度为75.76%。D=0.46时,聚类分析结果可以将供试材料分为2个类群,即子实体形态为小漏斗型、柄较长的菌株聚为一类,子实体形态为手掌或马蹄型、柄很短的菌株聚为一类。揭示了白灵侧耳菌株间的亲缘关系。
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| 关 键 词： | 白灵侧耳 RAPD 遗传多样性 聚类分析 |
| 文章编号： | 1008-0384(2006)04-0346-04 |
| 修稿时间： | 2006年4月6日 |
RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains |
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| HU Run-fang,XUE Zhu-zheng,TANG Yong-hui.RAPD analysis on Pleurotus nebrodensis (Inzengae) QuèL. strains[J].Fujian Journal of Agricultural Sciences,2006,21(4):346-349. |
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| Authors: | HU Run-fang XUE Zhu-zheng TANG Yong-hui |
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| Abstract: | Total genome DNA of P.nebrodensis mycelium was extracted by CTAB and the genetic relationships among 13 P.nebrodensis strains were analyzed by RAPD.11 primers of 80 random primers produced stable results.Among 66 bands obtained from 11 primers,50 bands were polymorphic bands which occupied 75.76% of the total amplified bands.Cluster analysis showed that the materials could be divided into 2 groups at the level of D=0.46,which evidently showed that the funnel-shaped fruitbody with a longer stem strains were grouped together,the horseshoe or palm-shaped fruitbody with a very short stem strains were grouped together.The cluster analysis of varieties revealed the relationships among P.nebrodensis strains. |
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| Keywords: | RAPD |
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