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| 猪链球菌对氟喹诺酮类药物的耐药性与靶位突变相关性 | |
| 引用本文： | 芮萍,马增军,段玲欣,刘谢荣,倪静,沈建忠.猪链球菌对氟喹诺酮类药物的耐药性与靶位突变相关性[J].畜牧兽医学报,2011,42(4). |
| 作者姓名： | 芮萍 马增军 段玲欣 刘谢荣 倪静 沈建忠 |
| 作者单位： | 1. 中国农业大学动物医学院,北京,100193;河北科技师范学院,河北省预防兽医学重点实验室,秦皇岛,066004 2. 河北科技师范学院,河北省预防兽医学重点实验室,秦皇岛,066004 3. 中国农业大学动物医学院,北京,100193 |
| 基金项目： | 河北省自然基金资助项目，河北省科技厅科技支撑项目，河北省教育厅计划项目 |
| 摘 要： | 旨在了解猪链球菌对氟喹诺酮类药物耐药性与parC、gyrA基因突变的相关性,通过微量稀释法测定34株猪链球菌对4种氟喹诺酮类药物的MIC值,采用PCR方法扩增并测序分析了临床分离的猪链球菌对氟唪诺酮类约物10株耐药株和9株敏感株的parC和gyrA基因喹诺酮耐药决定区(QRDRs).在氟喹诺酮类药物耐药菌株parC基因QRDRs发生Ser79→Phe、Arg 87→Leu的氨基酸突变,在4株高度耐药菌株gyrA基因QRDRs发生Arg66→Ser,Ser81→Arg氨基酸突变;当菌株对氟喹诺酮类药物敏感时,parC和gyrA基因的QRDR区均未有突变;而当MIC≥32 μg·L-1 时,parC的氨基酸发生了 Ser79→Phe的突变,同时发生gyrA氨基酸Arg66→Ser,Set81→Arg突变.结果表明,猪链球菌对氟喹诺酮类药物低水平类耐药是由parC单一位点突变引起,而高水平耐药是由parC和gyrA双位点突变引起. |
| 关 键 词： | 猪链球菌 氟喹诺酮类药物 parC基因 gyrA基因 |
Relationship between Fluoroquinolone Resistance and Gene Mutation in streptococcus suis |
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| RUI Ping,MA Zeng-jun,DUAN Ling-xin,LIU Xie-rong,NI Jing,SHEN Jian-zhong.Relationship between Fluoroquinolone Resistance and Gene Mutation in streptococcus suis[J].Acta Veterinaria et Zootechnica Sinica,2011,42(4). | |
| Authors: | RUI Ping MA Zeng-jun DUAN Ling-xin LIU Xie-rong NI Jing SHEN Jian-zhong |
| Institution: | RUI Ping1,2,MA Zeng-jun2,DUAN Ling-xin2,LIU Xie-rong2,NI Jing2,SHEN Jian-zhong1 (1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.Key Laboratory of Preventive Veterinary Medicine of Hebei,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China) |
| Abstract: | To detect the relationship between fluoroquinolone resistance and gyrA and parC gene mutation in Streptococcus suis.The MIC of 34 strains of Streptococcus suis were determined by microdilution.The genes encoding the quinolone-resistance determining region(QRDRs) of parC and gyrA in fluoroquinolone-susceptible and-resistant Streptococcus suis clinical isolates were identified and sequenced.Ser to Phe and Arg to Leu mutation at position 79 and 87 of the parC gene was detected in fluoroquinolone resistance S.s... |
| Keywords: | Streptococcus suis fluoroquinolone parC gene gyrA gene |
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