| 中华鲟早期发育中17β-雌二醇和睾酮水平变化的初步观察 |
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| 引用本文： | 吴湘香,陈细华,刘鉴毅,危起伟.中华鲟早期发育中17β-雌二醇和睾酮水平变化的初步观察[J].淡水渔业,2007,37(2):3-7. |
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| 作者姓名： | 吴湘香 陈细华 刘鉴毅 危起伟 |
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| 作者单位： | 1. 农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所,湖北荆州,434000;江西农业大学,南昌,330045
2. 农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所,湖北荆州,434000;中国水产科学研究院淡水渔业研究中心,江苏无锡,214081 |
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| 基金项目： | 国家自然科学基金，中国水产科学研究院水产种质资源与养殖技术实验室开放课题 |
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| 摘 要： | 采用放射免疫分析测定法(RIA)对人工繁殖的中华鲟(Acipenser sinensis)早期发育阶段中17β-雌二醇(17β-estradiol,E2)和睾酮(testosterone,T)的整体水平进行了测定。实验共测定了4批次(分别来自4尾雌鲟)受精卵及仔鱼的E2和T水平,结果表明:中华鲟胚胎发育期间E2水平在小范围内波动,T水平除胚胎发育早期出现上升外总体呈下降趋势;仔鱼出膜后E2水平开始逐渐上升,T水平是先降低后又逐渐升高。其中第1批次的测定结果(单位为pg/gtissue)为:胚胎发育期间E2水平在1.37~7.38之间波动,仔鱼出膜后3d为5.21,出膜后9d为37.26;胚胎发育期间T水平最低为293.58,最高为1420.41,仔鱼出膜后4d为117.96,出膜后7d为27.25,出膜后9d为148.48。对于仔鱼出膜后体内高水平的E2和T的来源,推测其可能与仔鱼自身合成性类固醇激素有关。另外,本实验中还发现,质量较差的卵子T水平明显低于质量较好者。
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| 关 键 词： | 中华鲟(Acipenser sinensis) 早期发育 17β-雌二醇 睾酮 |
| 文章编号： | 1000-6907-(2007)02-0003-05 |
| 修稿时间： | 2006-11-21 |
Variation in the Levels of 17β-Estradiol and Testosterone during Early Development of Chinese Sturgeon(Acipenser sinensis) |
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| WU Xiang-xiang,CHEN Xi-hua,LIU Jian-yi,WEI Qi-wei.Variation in the Levels of 17β-Estradiol and Testosterone during Early Development of Chinese Sturgeon(Acipenser sinensis)[J].Freshwater Fisheries,2007,37(2):3-7. |
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| Authors: | WU Xiang-xiang CHEN Xi-hua LIU Jian-yi WEI Qi-wei |
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| Abstract: | The Chinese sturgeon,Acipenser sinensis Gray,is a large anadromous fish.The fish is listed in the National GlassⅠProtected Animals,and the artificial spawning has been performed.In the experiment,the levels of 17β-estradiol(E2)and testosterone(T)during the early development of Chinese sturgeon were measured by radioimmunoassay(RIA).The levels of E2 and T of four batches of eggs and larvae were measured.The results showed that the level of E2 during embryonic development changed a little,while the level of T descended all through except for the early embryogenesis stage.After the larvae were hatched out,the level of E2 increased,but there was a decline of T level before it increased.The levels of E2 and T for one batch of eggs and larvae were as follows.The unit of levels of E2 and T were expressed by pg/g tissue.During the embryonic development,the level of E2 fluctuated between 1.37～7.38.The level of E2 was 5.21 at three days after larvae hatching,and 37.26 at nine days after larvae hatching.The lowest and highest levels of T were 293.58 and 1420.41 respectively during the embryonic development.When the larvae were out,the level of T was 117.96 at four days after hatching,27.25 at seven days after hatching,and 148.48 at nine days after hatching.The high levels of E2 and T in the larvae may be related with the sex steroids produced by the larvae.In addition,the result showed that the level of T in high-quality eggs was higher than that in the low-quality eggs. |
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| Keywords: | Acipenser sinensis early development 17β-estradiol testosterone |
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