| 产耐热纤维素酶菌株的筛选及其酶学性质研究 |
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| 引用本文： | 游庆红,尹秀莲.产耐热纤维素酶菌株的筛选及其酶学性质研究[J].安徽农业科学,2010,38(7):3331-3332. |
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| 作者姓名： | 游庆红 尹秀莲 |
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| 作者单位： | 淮阴工学院生命科学与化学工程学院,江苏淮安,223001;淮阴工学院生命科学与化学工程学院,江苏淮安,223001 |
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| 基金项目： | 淮阴工学院青年科学基金项目(HGC0918) |
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| 摘 要： | 目的]筛选产耐热纤维素酶菌株,并对其酶学性质进行研究。方法]分离筛选产耐热纤维素酶的菌株,并用菌种鉴定试剂盒进行鉴定。对产耐热纤维素酶的菌株的酶学性质进行研究。结果]从江苏淮安土壤中分离出1株产耐热纤维素酶的细菌,将其命名为H3,经菌种鉴定试剂盒初步鉴定为枯草芽孢杆菌。该酶最适反应温度为60℃,最适反应pH值为5.5,Cu^2＋、Mn^2＋对酶活性有一定的激活作用,Mg^2＋、Zn^2＋对酶活性有一定的抑制作用。结论]该研究为降低纤维素酶生产成本以及推动生物质乙醇的发展提供了科学依据。
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| 关 键 词： | 纤维素酶 细菌 枯草芽孢杆菌 酶学性质 |
Screening of Strains Producing Thermophilic Cellulose and Research on Its Enzymatic Characteristics |
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| YOU Qing-hong et al.Screening of Strains Producing Thermophilic Cellulose and Research on Its Enzymatic Characteristics[J].Journal of Anhui Agricultural Sciences,2010,38(7):3331-3332. |
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| Authors: | YOU Qing-hong |
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| Institution: | College of Life Sciences and Chemical Engineering;Huaiyin Institute of Technology;Huaian;Jiangsu 223001 |
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| Abstract: | Objective] The research aimed to screen the strains producing thermophilic cellulose and study its enzymatic characteristics.Method] The strains producing thermophilic cellulose were identified by strains identification kit.Then its enzymatic characteristics were studied.Method] A bacterium producing thermophilic cellulose was separated from Huaian's soil of Jiangsu Province,which was named as H3 and was identified as Bacillus subtilis by strains identification kit.The optimal reaction temperature and pH... |
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| Keywords: | Cellulose Bacterium Bacillus subtilis Enzymatic characteristics |
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