| 梅花EST SSR与Genomic SSR的比较研究 |
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| 引用本文： | 吴根松,张启翔,程汉武,孙丽丹,贺 丹.梅花EST SSR与Genomic SSR的比较研究[J].西北农业学报,2011,20(10):120-125. |
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| 作者姓名： | 吴根松 张启翔 程汉武 孙丽丹 贺 丹 |
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| 作者单位： | 北京林业大学园林学院国家花卉工程技术研究中心;中国梅花研究中心; |
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| 基金项目： | 公益性行业科研专项(201004012); 北京市教育委员会共建项目:梅花远缘杂交新品种选育及核心种质繁殖与示范、云南丽江照水梅良种高技术产业化示范工程 |
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| 摘 要： | 利用EST-SSR与Genomic-SSR标记技术对中国12个梅花品种的遗传多样性进行比较分析。结果表明,EST-SSR检测到的等位基因数、有效等位基因数、Shannon指数和PIC指数的平均值分别是3.9、2.4、0.9和0.4;Genomic-SSR检测到的等位基因数、有效等位基因数、Shannon指数和PIC指数的平均值分别是6.0、4.1、1.5和0.7,聚类结果表明,EST-SSR和Genomic-SSR均能有效鉴别所有的品种材料。这为梅花SSR分子标记的开发和应用奠定基础。
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| 关 键 词： | 梅花 EST-SSR Genomic-SSR |
Comparative Study of EST SSR and Genomic SSR in Prunus mume Sieb. et Zucc |
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| WU Gensong,ZHANG Qixiang,CHENG Hanwu,SUN Lidan and HE Dan.Comparative Study of EST SSR and Genomic SSR in Prunus mume Sieb. et Zucc[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(10):120-125. |
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| Authors: | WU Gensong ZHANG Qixiang CHENG Hanwu SUN Lidan and HE Dan |
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| Institution: | WU Gensong1,ZHANG Qixiang1,CHENG Hanwu2,SUN Lidan1 and HE Dan1(1.National Engineering Research Centre for Floriculture,College of Landscape Architecture,Beijing Forestry University,Beijing100083,China,2.Mei Flower Research Centre of China,Wuhan 430074,China) |
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| Abstract: | The genetic differences among 12 cultivars of mei(Prunus mume Sieb.et Zucc) were compared using EST-SSR and Genomic-SSR.The results showed that the average of number of alleles,effective number of alleles,the Shannon index and the polymorphic information content(PIC) value detected by EST-SSR was respectively 3.9,2.4,0.9,and 0.4,respectively.The average of number of alleles,effective number of alleles,the Shannon Index and the polymorphic information content(PIC) value detected by Genomic-SSR was respective... |
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| Keywords: | Mei(Prunus mume Sieb et Zucc) EST-SSR Genomic-SSR |
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