| 香果树带芽茎段不定芽高频增殖的优化 |
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| 引用本文： | 洪森荣,尹明华.香果树带芽茎段不定芽高频增殖的优化[J].核农学报,2010,24(3):532-536. |
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| 作者姓名： | 洪森荣 尹明华 |
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| 作者单位： | 上饶师范学院生命科学学院,江西上饶,334001 |
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| 基金项目： | 江西省教育厅青年科技项目,上饶师范学院2009-2010年度院级科技项目 |
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| 摘 要： | 以香果树(Emmenopterys henryi Oliv.)带芽茎段为外植体,以MS为基本培养基,采用正交试验和单因子试验方法研究了不同因素对带芽茎段不定芽增殖的影响。结果表明:香果树带芽茎段不定芽增殖的最佳培养基为MS+KT2.0mg/L+6-BA2.0mg/L+NAA0.1mg/L,最佳蔗糖和琼脂浓度分别为40和7.5g/L,最佳pH值6、培养温度25℃,培养条件为光培养。证明利用组织培养技术能够实现香果树带芽茎段不定芽的高频增殖,为珍稀濒危植物香果树的快速繁殖提供了一条简单.有效的途径。
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| 关 键 词： | 香果树 带芽茎段 不定芽 高频增殖 培养因素优化 |
THE OPTIMIZATION FOR HIGH-FREQUENCY PROLIFERATION OF ADVENTITIOUS BUDS OF Emmenopterys henryi Oliv. |
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| HONG Sen-rong,YIN Ming-hua.THE OPTIMIZATION FOR HIGH-FREQUENCY PROLIFERATION OF ADVENTITIOUS BUDS OF Emmenopterys henryi Oliv.[J].Acta Agriculturae Nucleatae Sinica,2010,24(3):532-536. |
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| Authors: | HONG Sen-rong YIN Ming-hua |
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| Institution: | College of Life Sciences, Shangrao Normal University, Shangrao,Jiangxi 334001 |
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| Abstract: | The effect of different factors on proliferation of Emmenopterys henryi Oliv. adventitious buds was studied using orthogonal and single-factor experimental method. The results showed that the best medium for the proliferation of Emmenopterys henryi Oliv. stem was MS+KT 2.0mg/L+6-BA 2.0mg/L+NAA 0.1mg/L, the best sugar and agar concentration were 40 and 7.5g/L, respectively, the appropriate pH value, temperature and culture conditions were 6, 25℃ and light culture, respectively. The research proved that high-frequency proliferation of E. henryi Oliv. adventitious buds could be achieved by tissue culture techniques, which provided a simple and effective way for rapid proliferation of the rare and endangered plants. |
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| Keywords: | Emmenopterys henryi Oliv " target="_blank">zz')" href="#"> Emmenopterys henryi Oliv stem with a bud adventitious buds high-frequency proliferation optimization of culture factors |
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