| 玉竹提取物A对小鼠免疫性肝损伤治疗作用的研究 |
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| 引用本文： | 赵良中,时文艳,鞠晓红,李妍.玉竹提取物A对小鼠免疫性肝损伤治疗作用的研究[J].中国畜牧兽医,2010,37(3):160-163. |
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| 作者姓名： | 赵良中 时文艳 鞠晓红 李妍 |
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| 作者单位： | （吉林医药学院病原教研室，吉林 132013） |
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| 摘 要： | 本试验旨在研究玉竹提取物A(extraction A of polygonatum odoratum,EA-PAOA)对刀豆蛋白A(ConA)诱导的小鼠免疫性肝损伤的治疗作用,探讨其发挥此作用的可能机制。将昆明小鼠随机分成正常对照组、模型对照组、试验1组(EA-PAOA 0.5 g/kg)、试验2组(EA-PAOA 1 g/kg)、试验3组(EA-PAOA 2 g/kg)。除正常对照组外,其余各组均尾静脉注射20mg/kg的ConA 0.5 mL,每天06∶00注射1次,连续5 d。每天注射ConA 2 h后,EA-PAOA 3个试验组分别腹腔注射不同浓度的EA-PAOA 0.5 mL,每天3次,共4 d,第5天只在注射ConA后2 h注射1次EA-PAOA。末次注射EA-PAOA后6 h,摘眼球取血低温保存,待测谷丙转氨酶活性(ALT)、3个细胞因子;取肝待行肝脏组织病理学检查注射;取脾待行淋巴细胞增殖试验(MTT法)和流式细胞术以检测T淋巴细胞亚群。结果显示,EA-PAOA 3个试验组血清中,ALT、IFN-γ、TNF-α水平明显降低,IL-10水平明显升高,能显著抑制T淋巴细胞的转化增殖,与模型对照组相比差异极显著(P0.01);但流式细胞术检测T淋巴细胞亚群发现,5组CD4+T和CD8+T淋巴细胞的数量和比例并无明显差别,都与正常对照组无显著差异;EA-PAOA 3个试验组肝脏组织病理改变较模型对照组明显改善。表明EA-PAOA可能是通过抑制T淋巴细胞的转化增殖,抑制其活性,调节Th1和Th2的数量及比例,降低血清中IFN-γ、TNF-α水平,提高IL-10水平,以此发挥其治疗免疫性肝损伤的功能。
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| 关 键 词： | 免疫性肝损伤 刀豆蛋白A 玉竹提取物A 淋巴细胞转化增殖 |
The Therapeutic Effects of EA-PAOA on Liver Injury Induced by Concanavalin A in Kunming Mice |
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| ZHAO Liang-zhong,SHI Wen-yan,JU Xiao-hong,LI Yan.The Therapeutic Effects of EA-PAOA on Liver Injury Induced by Concanavalin A in Kunming Mice[J].China Animal Husbandry & Veterinary Medicine,2010,37(3):160-163. |
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| Authors: | ZHAO Liang-zhong SHI Wen-yan JU Xiao-hong LI Yan |
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| Institution: | (Department of Pathogen Biology, Jilin Medical College, Jilin 132013, China) |
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| Abstract: | The objective of this paper was to investigate the therapeutic effects of EA-PAOA on the immune hepatic injury in Kunming mice and study its therapeutic mechanisms.Kunming mice were divided into five groups,which were normal control,model control,test 1(EA-PAOA 0.5 g/kg),test 2(EA-PAOA 1 g/kg),test 3(EA-PAOA 2 g/kg).Model control and three test groups,respectively,received intravenous injections of Concanavalin A(20 mg/kg) 0.5 mL,once one day,five days in all.Two hours after the first injection of Concanava... |
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| Keywords: | liver injury concanavalin A EA-PAoA T-cell proliferaction activity |
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