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| 组培枣苗基因组DNA提取方法及其含量的比较 | |
| 引用本文： | 陈宗礼,陈昱宇,王睿婷,陈国梁,齐向英.组培枣苗基因组DNA提取方法及其含量的比较[J].广西农业生物科学,2011,30(1):110-116. |
| 作者姓名： | 陈宗礼 陈昱宇 王睿婷 陈国梁 齐向英 |
| 作者单位： | 1. 陕西省区域生物资源保育与利用工程技术研究中心,延安,716000 2. 延安市公安局,延安,716000 3. 延安大学生命科学学院,延安,716000 |
| 基金项目： | 陕西省自然科学基金项目(S2009JC970); 陕西省重大科技专项(2006KZ05-G4); 陕西省教育厅重点实验室项目(05JS39)共同资助 |
| 摘 要： | 本文以组培二倍体木枣、四倍体变异苗和二倍体京枣苗为供试材料,采用琼脂糖凝胶电泳和紫外分光光度检测技术,研究了不同提取方法对其叶片基因组DNA的提取效果。结果表明,CTAB法提取枣叶片DNA的效果优于SDS法,表现为蛋白质杂质干扰少,DNA结构完整、纯度与得率高;用不同方法提取的样液中DNA片段的大小和分布是不同的,以改进的CTAB法提取的样液中大片段的DNA分子较多,所以在其底部分布较多,而用SDS法提取的样液中小片段的DNA分子较多,故在其上部分布较多;电泳时不同的加样量获得的DNA带也有较大差异,试验选出CTAB法提取样液的适宜的加样量为5μL（7.485μg）,SDS法提取样液的适宜的加样量为4μL（5.988μg）;枣品种间DNA的含量明显不同,木枣四倍体的DNA含量（3.074μg/μL）相当于组培木枣二倍体（1.497μg/μL）的2倍,也明显地高于京枣二倍体（2.182μg/μL）。这些结果为枣树遗传种质资源的研究奠定了一定的科学与技术基础。 |
| 关 键 词： | 组培枣 基因组DNA 提取方法 DNA含量 |
Comparison of the Extracting Method and Content of Genomics DNA on Tissue-cultured Jujube Seedling |
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| Chen Zongli , Chen Yuyu Wang Ruiting Chen Guoliang , Qi Xiangying , Shaanxi Engineering & Technological Research Center for Conservation & Utilization of Regional Biological Resources,Yan'an, College of Life Science,Yan'an University, The Public Security Bureau in Yan'an.Comparison of the Extracting Method and Content of Genomics DNA on Tissue-cultured Jujube Seedling[J].Journal of Guangxi Agricultural and Biological Science,2011,30(1):110-116. | |
| Authors: | Chen Zongli Chen Yuyu Wang Ruiting Chen Guoliang Qi Xiangying Shaanxi Engineering & Technological Research Center for Conservation & Utilization of Regional Biological Resources Yan'an College of Life Science Yan'an University The Public Security Bureau in Yan'an |
| Institution: | Chen Zongli 1,2* Chen Yuyu 3 Wang Ruiting 2 Chen Guoliang 1,2 Qi Xiangying 1,2 1 Shaanxi Engineering & Technological Research Center for Conservation & Utilization of Regional Biological Resources,Yan'an,716000,2 College of Life Science,Yan'an University,3 The Public Security Bureau in Yan'an |
| Abstract: | In this paper, we took the tissue-cultured diploid Mu-Zao, Mu-Zao tetraploid variance seedling and jujube Jing-Zao diploid seedling as the experimental materials to research different extraction methods on its genomic DNA extraction effect by using the agarose gel electrophoresis and UV detection technology. The results showed that the genomic DNA extraction effect of tissue-cultured jujube seedlings was the CTAB method better than the SDS method, performance for protein impurities interference less, DNA st... |
| Keywords: | Tissue-cultured jujube Genomics DNA Extracting method DNA content |
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