| 三种人参属植物的EST-SSR信息分析及其在三七中的应用 |
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| 引用本文： | 杨维泽,金航,崔秀明,沈涛,杨美权,张金渝.三种人参属植物的EST-SSR信息分析及其在三七中的应用[J].广西农业生物科学,2011,30(1):62-71. |
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| 作者姓名： | 杨维泽 金航 崔秀明 沈涛 杨美权 张金渝 |
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| 作者单位： | 1. 云南省农业科学院药用植物研究所,昆明,650223
2. 文山三七科学院,文山,663000 |
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| 基金项目： | 国家“十一五”科技支撑项目(2006BAI06A12-13); 云南省自然科学基金项目(2007C136M)共同资助 |
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| 摘 要： | 为了解人参属植物EST中SSR分布特点及其在三七SSR标记中的应用,利用生物信息学方法,用primer3软件对dbEST数据库中人参属植物人参、西洋参和三七的EST序列进行搜索,发现人参属植物EST-SSR出现频率为11.54%,平均每4.39kb出现1个SSR。人参属植物单核苷酸重复基元占主导地位,其次为三核苷酸重复基元和二核苷酸重复基元,分别占总SSR的54.48%、17.31%和16.36%。人参属EST-SSR的优势类型为A/T和AT/TA,分别占54.73%和8.21%。根据人参属植物EST中的SSR设计48对引物,在合适的PCR扩增体系下,用5个三七样品的DNA为模板进行PCR扩增,引物有效扩增率85.42%,其中多态性引物占可扩增引物的70.73%。结果表明,利用人参属EST序列开发三七EST-SSR标记是可行的。
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| 关 键 词： | 人参属 三七 EST-SSR |
Analysis on EST-SSR of Three Species in Genus Panax and the Application in Panax notoginseng |
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| Yang Weize , Jin Hang , Cui Xiuming Shen Tao Yang Meiquan Zhang Jinyu.Analysis on EST-SSR of Three Species in Genus Panax and the Application in Panax notoginseng[J].Journal of Guangxi Agricultural and Biological Science,2011,30(1):62-71. |
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| Authors: | Yang Weize Jin Hang Cui Xiuming Shen Tao Yang Meiquan Zhang Jinyu |
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| Institution: | Yang Weize 1,2 Jin Hang 1,2 Cui Xiuming 3 Shen Tao 1 Yang Meiquan 1 Zhang Jinyu 1,2* 1 Institute of Medicinal Plant,Yunnan Academy of Agricultural Sciences,Kunming,650223,2 Faculty of Life Science and Technology,Kunming University Science and Technology,650091,3 Wenshan Sanqi Research Institute,Wenshan,663000 |
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| Abstract: | In order to know the peculiarity of EST-SSR in three species of genus Panax and using in Panax notoginseng, with bioinformatics method, the EST sequences of Panax ginseng C. A. Mey., Panax quinque foliusm L. and Panax notoginseng (Burk) F. H. Chen were searched in dbEST database by using software primer 3. The frequency of EST-SSR was 11.54%, and the average distance between SSR was 4.39 kb. Mononucleotide repeats, accounting for 54.48%, was dominant in all SSR, followed by trinucleotide and dinucleotide re... |
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| Keywords: | Genus Panax Panax notoginseng EST-SSR |
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