| 金属硫蛋白在大鼠全脑缺血再灌注损伤中的神经保护作用 |
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| 引用本文： | 刘学忠,崔旭,王捍东,王宗元.金属硫蛋白在大鼠全脑缺血再灌注损伤中的神经保护作用[J].畜牧兽医学报,2004,35(1):42-45. |
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| 作者姓名： | 刘学忠 崔旭 王捍东 王宗元 |
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| 作者单位： | 1. 扬州大学畜牧兽医学院动物医学系,扬州,225009
2. 解放军总医院老年医学研究所,北京,100853 |
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| 基金项目： | 国家重点基础研究发展规划项目(G2000057000)，扬州大学科研基金项目(3550)资助。 |
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| 摘 要： | 采用股动脉放血并双侧颈总动脉夹闭的全脑缺血再灌注模型,测定缺血10min再灌注后6h、1d、3d、5d脑组织中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH Px)活力及丙二醛(MDA)含量,以探讨金属硫蛋白-1(MT 1)对实验性脑缺血再灌注的神经保护作用。结果表明:在对应的各时间相,老年组SOD及GSH Px的活性均显著低于青年组(P<0 05);MDA水平显著高于青年组(P<0 05);而MT-1可显著提高SOD及GSH Px的活性、降低MDA的水平(P<0 01)。本实验证明MT 1对脑缺血再灌注有明显神经保护作用。
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| 关 键 词： | 金属硫蛋白 大鼠 脑缺血 再灌注损伤 神经保护 |
| 文章编号： | 0366-6964(2004)01-0042-04 |
Neuroprotective Effect of Metallothionein in Cerebral Ischemia-reperfusion Injury of Rats |
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| LIU Xue-zhong,CUI Xu,WANG Han-dong,WANG Zong-yuan.Neuroprotective Effect of Metallothionein in Cerebral Ischemia-reperfusion Injury of Rats[J].Acta Veterinaria et Zootechnica Sinica,2004,35(1):42-45. |
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| Authors: | LIU Xue-zhong CUI Xu WANG Han-dong WANG Zong-yuan |
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| Institution: | LIU Xue-zhong~1,CUI Xu~2,WANG Han-dong~1,WANG Zong-yuan~1 |
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| Abstract: | In order to study neuroprotective effect of metallothionein-1(MT-1) in cerebral ischemia-reperfusion(IR) injury,we used bilateral carotid arteries occlusion model of rats and analysised the changes of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) in the brain tissue at 6 h?1 d?3 d and 5 d after reperfusion following 10min forebrain ischemia.The results showed in predeterminate time,the activity of SOD and GSH-Px in rat brain tissues in old group was significantly lower than that in young group (P<0.05).The content of MDA was significantly higher than that in young group (P<0.05),and MT-1 could increase the activity of SOD and GSH-Px,halt lipid peroxidative reactions in brain (P<0.01).It has been tested that MT-1 can protect brain tissue from IR injury. |
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| Keywords: | Metallothionein Lipid peroxidation Cerebral ischemia Reperfusion) |
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