| RT-PCR方法检测烟草环斑病毒的研究 |
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| 引用本文： | 孔宝华,蔡 红,陈海如,唐 慧,沐咏民,刘进元.RT-PCR方法检测烟草环斑病毒的研究[J].云南农业大学学报,2001,16(1):13-15. |
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| 作者姓名： | 孔宝华 蔡 红 陈海如 唐 慧 沐咏民 刘进元 |
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| 作者单位： | [1]云南农业大学，云南省植物病理重点实验室，云南昆明650201 [2]昆明动植物检疫局，云南昆明650034 |
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| 基金项目： | 云南省院省校合作研究项目 |
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| 摘 要： | 根据烟草环斑病毒（TRSV）外壳蛋白基因非编序列设计的引物P1,P2,用感病及健康组织总RNA为模板,进行cDNA 合成和PCR试验,感病组织中扩增出了600 bp的目的片段,而健康组织中无此扩增带。摸索各项实验条件,建立了RT-PCR检测烟草环斑病毒（TRSV）的方法。
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| 关 键 词： | 烟草环斑病毒（TRSV） RT-PCR 检测 |
| 文章编号： | 1004-390X(2001)01-0013-03 |
| 收稿时间： | 2000-3-28 |
The Study on Detection
Tobacco Ring-spot Virus (TRSV) by RT-PCR |
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| KONG Bao hua ,CAI Hong ,CHEN Hai ru ,MU Yong ming ,TANG Hui ,LIU Jin yuan.The Study on Detection
Tobacco Ring-spot Virus (TRSV) by RT-PCR[J].Journal of Yunnan Agricultural University,2001,16(1):13-15. |
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| Authors: | KONG Bao hua CAI Hong CHEN Hai ru MU Yong ming TANG Hui LIU Jin yuan |
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| Institution: | KONG Bao hua 1,CAI Hong 1,CHEN Hai ru 1**,MU Yong ming 2,TANG Hui 1,LIU Jin yuan 3 |
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| Abstract: | A pairs of primers of P 1,P 2 were designed, corresponding the coat protein genes of TRSV. The cDNA was made, and PCR was gotten on, with total RNA of the infected tissue and health tissue. The target fragment about 600 bp was amplified from the infected sample, but not from health sample. More condition were tested, The method of detection of TRSV by RT-PCR was built. |
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| Keywords: | Tobacco ringspot virus (TRSV) RT-PCR Detection |
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